2y1t

X-ray diffraction
1.89Å resolution

Bacillus subtilis prophage dUTPase in complex with dUDP

Released:
Source organism: Bacillus subtilis

Function and Biology Details

Reaction catalysed:
dUTP + H(2)O = dUMP + diphosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SPbeta prophage-derived deoxyuridine 5'-triphosphate nucleotidohydrolase YosS Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 142 amino acids
Theoretical weight: 16.17 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34919 (Residues: 1-142; Coverage: 100%)
Gene names: BSU20020, yojU, yosS
Sequence domains: dUTPase
Structure domains: Deoxyuridine 5'-Triphosphate Nucleotidohydrolase; Chain A

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 99.515Å b: 99.338Å c: 99.306Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.142 0.14 0.166
Expression system: Escherichia coli