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Crystal structures and repair studies reveal the identity and the base-pairing properties of the UV-induced spore photoproduct DNA lesion.

Heil K, Kneuttinger AC, Schneider S, Lischke U, Carell T
Chemistry 17 9651-7 (2011)
Cited: 15 times
EuropePMC logo PMID: 21780197

Abstract

UV light is one of the major causes of DNA damage. In spore DNA, due to an unusual packing of the genetic material, a special spore photoproduct lesion (SP lesion) is formed, which is repaired by the enzyme spore photoproduct lyase (Spl), a radical S-adenosylmethionine (SAM) enzyme. We report here the synthesis and DNA incorporation of a DNA SP lesion analogue lacking the phosphodiester backbone. The oligonucleotides were used for repair studies and they were cocrystallized with a polymerase enzyme as a template to clarify the configuration of the SP lesion and to provide information about the base-pairing properties of the lesion. The structural analysis together with repair studies allowed us to clarify the identity of the preferentially repaired lesion diastereoisomer.

Reviews citing this publication (7)

  1. Radical S-adenosylmethionine enzymes. Broderick JB, Duffus BR, Duschene KS, Shepard EM. Chem Rev 114 4229-4317 (2014)
  2. Early events of DNA photodamage. Schreier WJ, Gilch P, Zinth W. Annu Rev Phys Chem 66 497-519 (2015)
  3. Emerging themes in radical SAM chemistry. Shisler KA, Broderick JB. Curr Opin Struct Biol 22 701-710 (2012)
  4. Spore photoproduct lyase: the known, the controversial, and the unknown. Yang L, Li L. J Biol Chem 290 4003-4009 (2015)
  5. Photochemistry and Photobiology of the Spore Photoproduct: A 50-Year Journey. Setlow P, Li L. Photochem Photobiol 91 1263-1290 (2015)
  6. Mechanistic studies of the radical SAM enzyme spore photoproduct lyase (SPL). Li L. Biochim Biophys Acta 1824 1264-1277 (2012)
  7. Recent advances in cyclonucleosides: C-cyclonucleosides and spore photoproducts in damaged DNA. Yoshimura Y, Takahata H. Molecules 17 11630-11654 (2012)

Articles citing this publication (8)

  1. Structural insights into recognition and repair of UV-DNA damage by Spore Photoproduct Lyase, a radical SAM enzyme. Benjdia A, Heil K, Barends TR, Carell T, Schlichting I. Nucleic Acids Res 40 9308-9318 (2012)
  2. Hydrogen transfer in SAM-mediated enzymatic radical reactions. Hioe J, Zipse H. Chemistry 18 16463-16472 (2012)
  3. Chemical syntheses of oligodeoxyribonucleotides containing spore photoproduct. Jian Y, Li L. J Org Chem 78 3021-3029 (2013)
  4. The enzyme-mediated direct reversal of a dithymine photoproduct in germinating endospores. Yang L, Li L. Int J Mol Sci 14 13137-13153 (2013)
  5. Combined Mössbauer spectroscopic, multi-edge X-ray absorption spectroscopic, and density functional theoretical study of the radical SAM enzyme spore photoproduct lyase. Silver SC, Gardenghi DJ, Naik SG, Shepard EM, Huynh BH, Szilagyi RK, Broderick JB. J Biol Inorg Chem 19 465-483 (2014)
  6. Solution phase dynamics of the DNA repair enzyme spore photoproduct lyase as probed by H/D exchange. Ghose S, Hilmer JK, Bothner B, Broderick JB. FEBS Lett 588 3023-3029 (2014)
  7. Unusually large deuterium discrimination during spore photoproduct formation. Ames DM, Lin G, Jian Y, Cadet J, Li L. J Org Chem 79 4843-4851 (2014)
  8. Spore photoproduct within DNA is a surprisingly poor substrate for its designated repair enzyme-The spore photoproduct lyase. Yang L, Jian Y, Setlow P, Li L. DNA Repair (Amst) 53 31-42 (2017)


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