X-ray diffraction
3Å resolution

The Structure of the 2:1 (Partially Occupied) Complex Between Stearoyl Acyl Carrier Protein Desaturase from Ricinus Communis (Castor Bean) and Acyl Carrier Protein.

Primary publication:
Remote control of regioselectivity in acyl-acyl carrier protein-desaturases.
Proc. Natl. Acad. Sci. U.S.A. 108 16594-9 (2011)
PMID: 21930947

Function and Biology Details

Reaction catalysed:
Stearoyl-[acyl-carrier protein] + 2 reduced ferredoxin [iron-sulfur] cluster + O(2) + 2 H(+) = oleoyl-[acyl-carrier protein] + 2 oxidized ferredoxin [iron-sulfur] cluster + 2 H(2)O
Biological process:
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 363 amino acids
Theoretical weight: 41.7 KDa
Source organism: Ricinus communis
Expression system: Escherichia coli
  • Canonical: P22337 (Residues: 34-396; Coverage: 92%)
Sequence domains: Fatty acid desaturase
Structure domains: Ribonucleotide Reductase, subunit A
Acyl carrier protein 1, chloroplastic Chain: D
Molecule details ›
Chain: D
Length: 82 amino acids
Theoretical weight: 8.92 KDa
Source organism: Spinacia oleracea
Expression system: Escherichia coli
  • Canonical: P07854 (Residues: 57-138; Coverage: 59%)
Gene name: ACL1.1
Sequence domains: Phosphopantetheine attachment site
Structure domains: ACP-like

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
Spacegroup: P3121
Unit cell:
a: 188.274Å b: 188.274Å c: 81.315Å
α: 90° β: 90° γ: 120°
R R work R free
0.244 0.243 0.277
Expression system: Escherichia coli