› 2xxj

X-ray diffraction
1.96Å resolution

Penta mutant of lactate dehydrogenase from Thermus thermophilus, ternary complex

Released:
DOI: 10.2210/pdb2xxj/pdb
PDB entry 2xxj coloured by chain, front view.
PDB entry 2xxj coloured by chain, side view.
PDB entry 2xxj coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Tickle J, de Mendoza Barbera E, Vellieux FMD

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178009 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 310 amino acids
Theoretical weight: 32.6 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5SJA1 (Residues: 1-310; Coverage: 100%)
Gene names: TTHA1113, ldh
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 2 x NAD
2 bound ligands:
Ligand SO4 2 x SO4
Ligand OXM 2 x OXM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 57.15Å b: 147.36Å c: 152.28Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.226
Expression system: Escherichia coli

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