› 2xs2

X-ray diffraction
1.35Å resolution

Crystal structure of the RRM domain of mouse Deleted in azoospermia-like in complex with RNA, UUGUUCUU

Released:
DOI: 10.2210/pdb2xs2/pdb
PDB entry 2xs2 coloured by chain, front view.
PDB entry 2xs2 coloured by chain, side view.
PDB entry 2xs2 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Kinked β-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL.
Jenkins HT, Malkova B, Edwards TA
Proc Natl Acad Sci U S A 108 18266-71 (2011)
PMID: 22021443

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179127 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Deleted in azoospermia-like Chain: A
Molecule details ›
Chain: A
Length: 102 amino acids
Theoretical weight: 11.58 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q64368 (Residues: 32-132; Coverage: 34%)
Gene names: Dazl, Dazl1, Dazla
Sequence domains: RNA recognition motif
Structure domains: Alpha-Beta Plaits
5'-R(*UP*UP*GP*UP*UP*CP*UP*U)-3' Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.44 KDa
Source organism: Mus musculus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: P212121
Unit cell:
a: 38.1Å b: 52.06Å c: 60.49Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.163 0.161 0.193
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

10 review citations

RRM-RNA recognition: NMR or crystallography…and new findings.
Daubner et al. (2013)
9 more

6 mentions without citation

Kinked β-strands mediate high-affinity recognition of mRNA targets by the germ-cell regulator DAZL.
Jenkins et al. (2011)
5 more