PDBe 2xqn

X-ray diffraction
2.62Å resolution

Complex of the 2nd and 3rd LIM domains of TES with the EVH1 DOMAIN of MENA and the N-Terminal domain of actin-like protein Arp7A

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Actin-like protein 7A Chain: A
Molecule details ›
Chain: A
Length: 65 amino acids
Theoretical weight: 7.02 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9Y615 (Residues: 1-65; Coverage: 15%)
Gene name: ACTL7A
Sequence domains: Actin-like protein 7A N-terminus
Protein enabled homolog Chain: M
Molecule details ›
Chain: M
Length: 119 amino acids
Theoretical weight: 13.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8N8S7 (Residues: 1-116; Coverage: 20%)
Gene names: ENAH, MENA
Sequence domains: WH1 domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)
Testin Chain: T
Molecule details ›
Chain: T
Length: 126 amino acids
Theoretical weight: 14.37 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UGI8 (Residues: 296-421; Coverage: 30%)
Gene name: TES
Sequence domains: LIM domain
Structure domains: Cysteine Rich Protein

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 37.829Å b: 86.791Å c: 115.266Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.215 0.268
Expression system: Escherichia coli