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DEINOCOCCUS RADIODURANS ISDRA2 TRANSPOSASE COMPLEXED WITH LEFT END RECOGNITION AND CLEAVAGE SITE AND ZN

X-ray diffraction
1.9Å resolution
PDB entry 2xqc coloured by chain, front view.
PDB entry 2xqc coloured by chain, side view.
PDB entry 2xqc coloured by chain, top view.
Source organism: Deinococcus radiodurans
Assembly composition:
hetero hexamer (preferred)
Entry contents: 1 distinct polypeptide molecule
2 distinct DNA molecules

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Assemblies

Assembly 1 (preferred)
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Assembly Name: ISDra2 transposase TnpA and DNA
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Multimeric state: hetero hexamer
Accessible surface area: 17571.81 Å2
Buried surface area: 13076.49 Å2
Dissociation area: 1,985.53 Å2
Dissociation energy (ΔGdiss): 36.65 kcal/mol
Dissociation entropy (TΔSdiss): 17.26 kcal/mol
Symmetry number: 2
PDBe Complex ID: PDB-CPX-115937

Macromolecules

ISDra2 transposase TnpA
Chains: A, D
Length: 140 amino acids
Theoretical weight: 16.14 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q7DF83 (Residues: 1-140; Coverage: 100%)
Gene names: DR_0667, DR_0979, DR_1382, DR_1592, DR_1652, DR_1932, DR_2323, orf1, tnpA, tnpB
Pfam: Transposase IS200 like
InterPro:
CATH: Transposase IS200-like
ISDra2 transposase TnpA in PDB entry 2xqc, assembly 1, front view.
ISDra2 transposase TnpA in PDB entry 2xqc, assembly 1, side view.
ISDra2 transposase TnpA in PDB entry 2xqc, assembly 1, top view.
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DNA chains B, E
Name: DRA2 TRANSPOSASE LEFT END RECOGNITION SEQUENCE
Representative chains: B, E
Source organism: Deinococcus radiodurans [1299]
Expression system: Not provided
Length: 27 nucleotides
Theoretical weight: 8.25 KDa
DRA2 TRANSPOSASE LEFT END RECOGNITION SEQUENCE in PDB entry 2xqc, assembly 1, front view.
DRA2 TRANSPOSASE LEFT END RECOGNITION SEQUENCE in PDB entry 2xqc, assembly 1, side view.
DRA2 TRANSPOSASE LEFT END RECOGNITION SEQUENCE in PDB entry 2xqc, assembly 1, top view.
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DNA chains C, F
Name: 5'-D(TP*TP*GP*AP*TP*GP)-3'
Representative chains: C, F
Source organism: Deinococcus radiodurans [1299]
Expression system: Not provided
Length: 6 nucleotides
Theoretical weight: 1.84 KDa
5'-D(TP*TP*GP*AP*TP*GP)-3' in PDB entry 2xqc, assembly 1, front view.
5'-D(TP*TP*GP*AP*TP*GP)-3' in PDB entry 2xqc, assembly 1, side view.
5'-D(TP*TP*GP*AP*TP*GP)-3' in PDB entry 2xqc, assembly 1, top view.
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