2xqc

X-ray diffraction
1.9Å resolution

DEINOCOCCUS RADIODURANS ISDRA2 TRANSPOSASE COMPLEXED WITH LEFT END RECOGNITION AND CLEAVAGE SITE AND ZN

Released:

Function and Biology Details

Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Y1_Tnp domain-containing protein Chains: A, D
Molecule details ›
Chains: A, D
Length: 140 amino acids
Theoretical weight: 16.14 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O83028 (Residues: 1-140; Coverage: 100%)
Gene name: tnpB
Sequence domains: Transposase IS200 like
Structure domains: Transposase IS200-like
DRA2 TRANSPOSASE LEFT END RECOGNITION SEQUENCE Chains: B, E
Molecule details ›
Chains: B, E
Length: 27 nucleotides
Theoretical weight: 8.25 KDa
Source organism: Deinococcus radiodurans
Expression system: Not provided
5'-D(TP*TP*GP*AP*TP*GP)-3' Chains: C, F
Molecule details ›
Chains: C, F
Length: 6 nucleotides
Theoretical weight: 1.84 KDa
Source organism: Deinococcus radiodurans
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: P212121
Unit cell:
a: 49.762Å b: 92.716Å c: 126.07Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.176 0.176 0.209
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided