› 2xod

X-ray diffraction
0.96Å resolution

Crystal structure of flavoprotein NrdI from Bacillus anthracis in the oxidised form

Released:
DOI: 10.2210/pdb2xod/pdb
PDB entry 2xod coloured by chain, front view.
PDB entry 2xod coloured by chain, side view.
PDB entry 2xod coloured by chain, top view.
Source organism: Bacillus anthracis
Primary publication:
High-resolution crystal structures of the flavoprotein NrdI in oxidized and reduced states--an unusual flavodoxin. Structural biology.
Johansson R, Torrents E, Lundin D, Sprenger J, Sahlin M, Sjöberg BM, Logan DT
FEBS J 277 4265-77 (2010)
PMID: 20831589

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-103755 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protein NrdI Chain: A
Molecule details ›
Chain: A
Length: 119 amino acids
Theoretical weight: 13.47 KDa
Source organism: Bacillus anthracis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A1T3V7R2 (Residues: 1-119; Coverage: 100%)
Gene names: GBAA_1369, nrdI
Sequence domains: NrdI Flavodoxin like
Structure domains: Rossmann fold

Ligands and Environments

3 bound ligands:
Ligand FMN 1 x FMN
Ligand ZN 1 x ZN
Ligand CAC 1 x CAC
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P212121
Unit cell:
a: 42.54Å b: 45.55Å c: 56.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.124 not available 0.151
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Class I ribonucleotide reductases: metallocofactor assembly and repair in vitro and in vivo.
Cotruvo et al. (2011)
2 more

4 mentions without citation

High-resolution crystal structures reveal a mixture of conformers of the Gly61-Asp62 peptide bond in an oxidized flavodoxin from Bacillus cereus.
Gudim et al. (2018)
3 more