› 2xmp

X-ray diffraction
2.5Å resolution

Crystal structure of trehalose synthase TreT mutant E326A from P. horishiki in complex with UDP

Released:
DOI: 10.2210/pdb2xmp/pdb
PDB 2xmp coloured by chain and viewed from the front.
PDB 2xmp coloured by chain and viewed from the side.
PDB 2xmp coloured by chain and viewed from the top.
Source organism: Pyrococcus horikoshii
Primary publication:
Structural insights on the new mechanism of trehalose synthesis by trehalose synthase TreT from Pyrococcus horikoshii.
Woo EJ, Ryu SI, Song HN, Jung TY, Yeon SM, Lee HA, Park BC, Park KH, Lee SB
J. Mol. Biol. 404 247-59 (2010)
PMID: 20888836

Function and Biology Details

Reaction catalysed: 
NDP-alpha-D-glucose + D-glucose = alpha,alpha-trehalose + NDP
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trehalose synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 416 amino acids
Theoretical weight: 48.18 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O58762 (Residues: 2-415; Coverage: 100%)
Gene names: PH1035, treT
Sequence domains: Glycosyl transferases group 1
Structure domains: Glycogen Phosphorylase B;

Ligands and Environments

1 bound ligand:
Ligand UDP 2 x UDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P21
Unit cell:
a: 80.874Å b: 63.017Å c: 91.107Å
α: 90° β: 98.84° γ: 90°
R-values:
R R work R free
0.209 0.209 0.271
Expression system: Escherichia coli

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Citations

10 citation in other articles

Domain organization of the polymerizing mannosyltransferases involved in synthesis of the Escherichia coli O8 and O9a lipopolysaccharide O-antigens.
Greenfield et al. (2012)
9 more

1 mention without citation

Self-association of a highly charged arginine-rich cell-penetrating peptide.
Tesei et al. (2017)