› 2xmm

X-ray diffraction
1.65Å resolution

Visualising the Metal-binding Versatility of Copper Trafficking Sites: H61Y Atx1 side-to-side

Released:
DOI: 10.2210/pdb2xmm/pdb
PDB entry 2xmm coloured by chain, front view.
PDB entry 2xmm coloured by chain, side view.
PDB entry 2xmm coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Visualizing the metal-binding versatility of copper trafficking sites .
Badarau A, Firbank SJ, McCarthy AA, Banfield MJ, Dennison C
Biochemistry 49 7798-810 (2010)
PMID: 20726513

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159886 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HMA domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 64 amino acids
Theoretical weight: 6.72 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P73213 (Residues: 1-64; Coverage: 100%)
Gene name: ssr2857
Sequence domains: Heavy-metal-associated domain
Structure domains: Alpha-Beta Plaits

Ligands and Environments

3 bound ligands:
Ligand CL 1 x CL
Ligand CU 4 x CU
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I03
Spacegroup: C2
Unit cell:
a: 62.63Å b: 41.4Å c: 55.58Å
α: 90° β: 106.76° γ: 90°
R-values:
R R work R free
0.167 0.165 0.2
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Single-molecule dynamics and mechanisms of metalloregulators and metallochaperones.
Chen et al. (2013)
1 more