2xmh

X-ray diffraction
2.4Å resolution

The X-ray structure of CTP:inositol-1-phosphate cytidylyltransferase from Archaeoglobus fulgidus

Released:

Function and Biology Details

Reactions catalysed:
CTP + 1L-myo-inositol 1-phosphate = diphosphate + CDP-1L-myo-inositol
CDP-1L-myo-inositol + 1L-myo-inositol 1-phosphate = CMP + bis(1L-myo-inositol) 3,1'-phosphate 1-phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bifunctional IPC transferase and DIPP synthase Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 232 amino acids
Theoretical weight: 26.29 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29976 (Residues: 55-286; Coverage: 47%)
Gene name: AF_0263
Sequence domains: MobA-like NTP transferase domain
Structure domains: Spore Coat Polysaccharide Biosynthesis Protein SpsA; Chain A

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21212
Unit cell:
a: 154.68Å b: 83.85Å c: 127.69Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.235 0.233 0.275
Expression system: Escherichia coli BL21(DE3)