› 2xl7

X-ray diffraction
2.4Å resolution

Structure and metal-loading of a soluble periplasm cupro-protein: Cu- CucA-closed (SeMet)

Released:
DOI: 10.2210/pdb2xl7/pdb
PDB entry 2xl7 coloured by chain, front view.
PDB entry 2xl7 coloured by chain, side view.
PDB entry 2xl7 coloured by chain, top view.
Source organism: Synechocystis sp. PCC 6803
Primary publication:
Structure and metal loading of a soluble periplasm cuproprotein.
Waldron KJ, Firbank SJ, Dainty SJ, Pérez-Rama M, Tottey S, Robinson NJ
J Biol Chem 285 32504-11 (2010)
PMID: 20702411

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-159902 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cupin type-1 domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 239 amino acids
Theoretical weight: 27.31 KDa
Source organism: Synechocystis sp. PCC 6803
Expression system: Escherichia coli
UniProt:
  • Canonical: P73600 (Residues: 31-268; Coverage: 89%)
Gene name: sll1785
Structure domains: Jelly Rolls

Ligands and Environments

3 bound ligands:
Ligand CU 2 x CU
Ligand URE 1 x URE
Ligand CL 1 x CL
1 modified residue:
Ligand MSE 9 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P6422
Unit cell:
a: 88.483Å b: 88.483Å c: 192.419Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.176 0.174 0.213
Expression system: Escherichia coli

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Citations

5 review citations

Metals in cyanobacteria: analysis of the copper, nickel, cobalt and arsenic homeostasis mechanisms.
Huertas et al. (2014)
4 more

2 mentions without citation

Metal preferences and metallation.
Foster et al. (2014)
1 more