Protein Data Bank in Europe

Hydrolysis of proteins in presence of ATP.

5-methyl-5,6,7,8-tetrahydromethanopterin + coenzyme M + 2 Na(+)(In) = 5,6,7,8-tetrahydromethanopterin + 2-(methylsulfanyl)ethane-1-sulfonate + 2 Na(+)(Out)

Acts on substrates that are at least partially unfolded. The cleavage site P1 residue is normally between a pair of hydrophobic residues, such as Val-|-Val

ATP + D-ribose = ADP + D-ribose 5-phosphate

Prephenate + NADP(+) = 4-hydroxyphenylpyruvate + CO(2) + NADPH

Cyclizes part of a (1->6)-alpha-D-glucan chain by formation of a (1->6)-alpha-D-glucosidic bond

Endonucleolytic cleavage of RNA, removing 21 and 42 nucleotides, respectively, from the 5'- and 3'-termini of a 5S-rRNA precursor.

S-adenosyl-L-methionine + adenine in DNA = S-adenosyl-L-homocysteine + N-6-methyladenine in DNA 

6-phospho-D-glucono-1,5-lactone + H(2)O = 6-phospho-D-gluconate

2'-deoxyribonucleoside diphosphate + thioredoxin disulfide + H(2)O = ribonucleoside diphosphate + thioredoxin

4 Fe(2+) + 4 H(+) + O(2) = 4 Fe(3+) + 2 H(2)O

4 benzenediol + O(2) = 4 benzosemiquinone + 2 H(2)O

L-serine + L-homocysteine = L-cystathionine + H(2)O

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO(2) + [acyl-carrier-protein]

Protein N(6)-(dihydrolipoyl)lysine + NAD(+) = protein N(6)-(lipoyl)lysine + NADH

Thioredoxin + NADP(+) = thioredoxin disulfide + NADPH

(1a) ATP + [RNA ligase]-L-lysine = [RNA ligase]-N(6)-(5'-adenylyl)-L-lysine + diphosphate

ATP + (d)CMP = ADP + (d)CDP

D-glucose 6-phosphate = D-fructose 6-phosphate

5,10-methylenetetrahydrofolate + dUMP = dihydrofolate + dTMP

5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate = (S)-allantoin + CO(2)

L-aspartate = D-aspartate

An L-amino acid = a D-amino acid

5,10-methylenetetrahydrofolate + glycine + H(2)O = tetrahydrofolate + L-serine

S-(hydroxymethyl)glutathione + NAD(P)(+) = S-formylglutathione + NAD(P)H

2-deoxy-D-ribose 5-phosphate = D-glyceraldehyde 3-phosphate + acetaldehyde

Methylamine + H(2)O + 2 oxidized [amicyanin] = formaldehyde + NH(3) + 2 reduced [amicyanin]

A 4-O-methyl-D-glucopyranuronate ester + H(2)O = 4-O-methyl-D-glucuronic acid + an alcohol

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

L-carnitine + NAD(P)H + O(2) = (3R)-3-hydroxy-4-oxobutanoate + trimethylamine + NAD(P)(+) + H(2)O

ATP + nucleoside diphosphate = ADP + nucleoside triphosphate

L-glutamate + H(2)O + NADP(+) = 2-oxoglutarate + NH(3) + NADPH

5-hydroxyisourate + H(2)O = 5-hydroxy-2-oxo-4-ureido-2,5-dihydro-1H-imidazole-5-carboxylate

Oxalate + O(2) + 2 H(+) = 2 CO(2) + H(2)O(2)

L-alanine + 3-oxopropanoate = pyruvate + beta-alanine

4-aminobutanoate + 2-oxoglutarate = succinate semialdehyde + L-glutamate

Betaine aldehyde + NAD(+) + H(2)O = betaine + NADH

Selective cleavage of Gln-|-Gly bond in the poliovirus polyprotein. In other picornavirus reactions Glu may be substituted for Gln, and Ser or Thr for Gly.

Selective cleavage of Tyr-|-Gly bond in picornavirus polyprotein.

D-erythro-1-(imidazol-4-yl)glycerol 3-phosphate = 3-(imidazol-4-yl)-2-oxopropyl phosphate + H(2)O

Tetradecanoyl-CoA + an N-terminal-glycyl-[protein] = CoA + an N-terminal-N-tetradecanoylglycyl-[protein]

(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O

Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.

(2E,6E)-farnesyl diphosphate = amorpha-4,11-diene + diphosphate

ATP + (L-Asp(4-L-Arg))(n) + L-Asp = ADP + phosphate + (L-Asp(4-L-Arg))(n)-L-Asp

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol

Hydrolysis of terminal, non-reducing branched (1->3)-alpha-D-galactosidic residues, producing free D-galactose

A beta-D-glucuronoside + H(2)O = D-glucuronate + an alcohol

1-(5-phospho-beta-D-ribosyl)-AMP + H(2)O = 1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans

Isovaleryl-CoA + electron-transfer flavoprotein = 3-methylcrotonyl-CoA + reduced electron-transfer flavoprotein

RH + [reduced NADPH--hemoprotein reductase] + O(2) = ROH + [oxidized NADPH--hemoprotein reductase] + H(2)O

8 reduced ferredoxin + 8 H(+) + N(2) + 16 ATP + 16 H(2)O = 8 oxidized ferredoxin + H(2) + 2 NH(3) + 16 ADP + 16 phosphate

S-adenosyl-L-methionine + a catechol = S-adenosyl-L-homocysteine + a guaiacol

ATP + H(2)O + 4 H(+)(Side 1) = ADP + phosphate + 4 H(+)(Side 2)

N-substituted aminoacyl-tRNA + H(2)O = N-substituted amino acid + tRNA

(1a) L-tyrosine + 1/2 O(2) = L-dopa

Endohydrolysis of (1->4)-beta-D-linkages between D-galactose 4-sulfate and 3,6-anhydro-D-galactose in kappa-carrageenans.

Urea + H(2)O = CO(2) + 2 NH(3)

(Ribonucleotide)(n)-2',3'-cyclic phosphate + H(2)O = (ribonucleotide)(n)-2'-phosphate

FAD + [protein]-L-threonine = [protein]-FMN-L-threonine + AMP

TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.

A (Z)-hexadec-9-enoyl-[acyl-carrier protein] + a malonyl-[acyl-carrier protein] = a (Z)-3-oxooctadec-11-enoyl-[acyl-carrier protein] + CO(2) + an [acyl-carrier protein]

(R)-10-hydroxystearate = oleate + H(2)O

NTP + H(2)O = NDP + phosphate

ATP + L-glutamate + NH(3) = ADP + phosphate + L-glutamine

L-methionine + thioredoxin disulfide + H(2)O = L-methionine (S)-S-oxide + thioredoxin

S-adenosyl-L-methionine + guanine(37) in tRNA = S-adenosyl-L-homocysteine + N(1)-methylguanine(37) in tRNA

RX + glutathione = HX + R-S-glutathione

Similar to that of papain.

(1a) L-methionine = methanethiol + 2-aminobut-2-enoate

Alpha-D-glucosamine 1-phosphate = D-glucosamine 6-phosphate

Hydrolysis of (1->4)-beta-D-glucosidic linkages in beta-D-glucans containing (1->3)- and (1->4)-bonds

2 H(2)O(2) = O(2) + 2 H(2)O

2 phenolic donor + H(2)O(2) = 2 phenoxyl radical of the donor + 2 H(2)O

(S)-3-hydroxyacyl-CoA + NAD(+) = 3-oxoacyl-CoA + NADH

D-fructose 1,6-bisphosphate + H(2)O = D-fructose 6-phosphate + phosphate

Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends

S-methyl-5'-thioadenosine + phosphate = adenine + S-methyl-5-thio-alpha-D-ribose 1-phosphate

S-adenosyl-L-homocysteine + H(2)O = L-homocysteine + adenosine

L-glutamate = 4-aminobutanoate + CO(2)

Prenyl phosphate + FMNH(2) = prenylated FMNH(2) + phosphate

D-glucose 6-phosphate + NADP(+) = 6-phospho-D-glucono-1,5-lactone + NADPH

6-phospho-D-gluconate + NADP(+) = D-ribulose 5-phosphate + CO(2) + NADPH

Acetyl-CoA + H(2)O + acetoacetyl-CoA = (S)-3-hydroxy-3-methylglutaryl-CoA + CoA

Methyl-CoM + CoB = CoM-S-S-CoB + methane

Beta-D-ribopyranose = beta-D-ribofuranose

ATP + pyridoxal = ADP + pyridoxal 5'-phosphate

Sucrose + (6)-beta-D-fructofuranosyl-(2->)(n) alpha-D-glucopyranoside = glucose + (6)-beta-D-fructofuranosyl-(2->)(n+1) alpha-D-glucopyranoside

Pyruvate + L-aspartate-4-semialdehyde = (4S)-4-hydroxy-2,3,4,5-tetrahydro-(2S)-dipicolinate + H(2)O

A pyrimidine nucleoside + H(2)O = D-ribose + a pyrimidine base

L-aspartate = fumarate + NH(3)

Acetyl-CoA + malonyl-[acyl-carrier-protein] = acetoacetyl-[acyl-carrier-protein] + CoA + CO(2) 

The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate

2-iminobutanoate + H(2)O = 2-oxobutanoate + NH(3)

Purine deoxynucleoside + phosphate = purine + 2'-deoxy-alpha-D-ribose 1-phosphate

Uridine + phosphate = uracil + alpha-D-ribose 1-phosphate 

ATP + a protein = ADP + a phosphoprotein

L-rhamnonate = 2-dehydro-3-deoxy-L-rhamnonate + H(2)O

UDP-N-acetyl-alpha-D-glucosamine + H(2)O = N-acetyl-D-mannosamine + UDP

H(2) + oxidized coenzyme F420 = reduced coenzyme F420

Chorismate = prephenate

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine

Nitric oxide + H(2)O + ferricytochrome c = nitrite + ferrocytochrome c + 2 H(+)

ATP + shikimate = ADP + shikimate 3-phosphate

An acyl-[acyl-carrier protein] + NADP(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADPH

Orotidine 5'-phosphate = UMP + CO(2)

N-acetyl-O-acetylneuraminate + H(2)O = N-acetylneuraminate + acetate

dTDP-4-dehydro-6-deoxy-alpha-D-glucose = dTDP-4-dehydro-beta-L-rhamnose

(3R)-3-hydroxyacyl-[acyl-carrier-protein] + NADP(+) = 3-oxoacyl-[acyl-carrier-protein] + NADPH

Aceneneuramate = N-acetyl-D-mannosamine + pyruvate

A beta-lactam + H(2)O = a substituted beta-amino acid

GDP-alpha-D-mannose = GDP-4-dehydro-alpha-D-rhamnose + H(2)O

Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)

Peptidylproline (omega=180) = peptidylproline (omega=0)

L-aspartate 4-semialdehyde + phosphate + NADP(+) = L-4-aspartyl phosphate + NADPH

2 superoxide + 2 H(+) = O(2) + H(2)O(2)

N,N-dimethylformamide + H(2)O = dimethylamine + formate

Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

L-histidinol + H(2)O + 2 NAD(+) = L-histidine + 2 NADH

(S)-lactate + NAD(+) = pyruvate + NADH

Adenine + H(2)O = hypoxanthine + NH(3)

Cytosine + H(2)O = uracil + NH(3)

2'-deoxycytidine + H(2)O = 2'-deoxyuridine + NH(3)

IMP + H(2)O = inosine + phosphate

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu)

ATP + L-tyrosine + tRNA(Tyr) = AMP + diphosphate + L-tyrosyl-tRNA(Tyr)

A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + UDP-N-acetyl-alpha-D-glucosamine = an [acyl-carrier-protein] + a UDP-3-O-(3-hydroxyacylyl)-N-acetyl-alpha-D-glucosamine

ATP + H(2)O + a folded polypeptide = ADP + phosphate + an unfolded polypeptide

ATP + H(2)O = ADP + phosphate

L-proline + NAD(P)(+) = 1-pyrroline-5-carboxylate + NAD(P)H

5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + NADPH

5,10-methylenetetrahydrofolate + NADP(+) = 5,10-methenyltetrahydrofolate + NADPH

5,10-methenyltetrahydrofolate + H(2)O = 10-formyltetrahydrofolate

A phosphate monoester + H(2)O = an alcohol + phosphate

4 sulfur + 4 H(2)O + O(2) = 2 H(2)S + 2 HSO(3)(-) + 2 H(+)

Cleavage of peptide bonds with very broad specificity.

3 malonyl-CoA + 4-coumaroyl-CoA = 4 CoA + naringenin chalcone + 3 CO(2)

Release of N-terminal proline from a peptide.

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.

1-(5-phospho-beta-D-ribosyl)-ATP + H(2)O = 1-(5-phospho-beta-D-ribosyl)-AMP + diphosphate

Diphosphate + H(2)O = 2 phosphate

An aliphatic amide = a nitrile + H(2)O

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

(1,4-alpha-D-galacturonosyl)(n+m) + H(2)O = (1,4-alpha-D-galacturonosyl)(n) + (1,4-alpha-D-galacturonosyl)(m)

Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins

Preferential cleavage: Tyr-|-Xaa, Trp-|-Xaa, Phe-|-Xaa, Leu-|-Xaa.

Formyl-L-methionyl peptide + H(2)O = formate + methionyl peptide

An acyl-[acyl-carrier protein] + NAD(+) = a trans-2,3-dehydroacyl-[acyl-carrier protein] + NADH

5-carboxyamino-1-(5-phospho-D-ribosyl)imidazole = 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate

Maltose = alpha,alpha-trehalose

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA

Endohydrolysis of RNA in RNA/DNA hybrids. Three different cleavage modes: 1. sequence-specific internal cleavage of RNA. Human immunodeficiency virus type 1 and Moloney murine leukemia virus enzymes prefer to cleave the RNA strand one nucleotide away from the RNA-DNA junction. 2. RNA 5'-end directed cleavage 13-19 nucleotides from the RNA end. 3. DNA 3'-end directed cleavage 15-20 nucleotides away from the primer terminus.

sn-glycerol 3-phosphate + O(2) = glycerone phosphate + H(2)O(2)

Protein tyrosine phosphate + H(2)O = protein tyrosine + phosphate

3-deoxy-D-manno-octulosonate 8-phosphate + H(2)O = 3-deoxy-D-manno-octulosonate + phosphate

2 3-phospho-D-glycerate + 2 H(+) = D-ribulose 1,5-bisphosphate + CO(2) + H(2)O

Hydrolysis of proteins to small peptides in the presence of ATP and magnesium. Alpha-Casein is the usual test substrate. In the absence of ATP, only oligopeptides shorter than five residues are hydrolyzed (such as succinyl-Leu-Tyr-|-NHMec; and Leu-Tyr-Leu-|-Tyr-Trp, in which cleavage of the -Tyr-|-Leu- and -Tyr-|-Trp bonds also occurs).

Selective hydrolysis of -Xaa-Xaa-|-Yaa- bonds in which each of the Xaa can be either Arg or Lys and Yaa can be either Ser or Ala.

Hydrolysis of four peptide bonds in the viral precursor polyprotein, commonly with Asp or Glu in the P6 position, Cys or Thr in P1 and Ser or Ala in P1'.

Acetyl-CoA + L-2,4-diaminobutanoate = CoA + N(4)-acetyl-L-2,4-diaminobutanoate

Hydrolyzes glutaminyl bonds, and activity is further restricted by preferences for the amino acids in P6 - P1' that vary with the species of potyvirus, e.g. Glu-Xaa-Xaa-Tyr-Xaa-Gln-|-(Ser or Gly) for the enzyme from tobacco etch virus. The natural substrate is the viral polyprotein, but other proteins and oligopeptides containing the appropriate consensus sequence are also cleaved.

Hydrolyzes a Gly-|-Gly bond at its own C-terminus, commonly in the sequence -Tyr-Xaa-Val-Gly-|-Gly, in the processing of the potyviral polyprotein.

6-carboxy-5,6,7,8-tetrahydropterin = 7-carboxy-7-carbaguanine + NH(3)

Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans

Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans

Endohydrolysis of (1->3)- or (1->4)-linkages in beta-D-glucans when the glucose residue whose reducing group is involved in the linkage to be hydrolyzed is itself substituted at C-3.

Endohydrolysis of (1->4)-alpha-D-glucosidic linkages in polysaccharides containing three or more (1->4)-alpha-linked D-glucose units

H(2)CO(3) = CO(2) + H(2)O

Inosine 5'-phosphate + NAD(+) + H(2)O = xanthosine 5'-phosphate + NADH

Endohydrolysis of the N-glycosidic bond at one specific adenosine on the 28S rRNA.

Acetyl-CoA + a 2-deoxystreptamine antibiotic = CoA + N(3)-acetyl-2-deoxystreptamine antibiotic

Acetyl-CoA + n malonyl-CoA + 2n NADPH = long-chain-acyl-CoA + n CoA + n CO(2) + 2n NADP(+)

1-(5-phospho-beta-D-ribosyl)-ATP + diphosphate = ATP + 5-phospho-alpha-D-ribose 1-diphosphate

An alpha-L-fucoside + H(2)O = L-fucose + an alcohol

Hydrolysis of terminal non-reducing N-acetyl-D-hexosamine residues in N-acetyl-beta-D-hexosaminides

(S)-lactaldehyde + NAD(+) + H(2)O = (S)-lactate + NADH

H(2) + A = AH(2)

ATP + (L-Asp(4-L-Arg))(n)-L-Asp + L-Arg = ADP + phosphate + (L-Asp(4-L-Arg))(n+1)

Acetyl-CoA + L-serine = CoA + O-acetyl-L-serine

L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H

Hydrolysis of terminal, non-reducing (1->4)-linked alpha-D-glucose residues with release of alpha-D-glucose

Hydrolysis of terminal, non-reducing beta-D-glucosyl residues with release of beta-D-glucose

Hydrolysis of terminal, non-reducing alpha-D-galactose residues in alpha-D-galactosides, including galactose oligosaccharides, galactomannans and galactolipids

Hydrolysis of terminal non-reducing beta-D-galactose residues in beta-D-galactosides

Quinol + 2 ferricytochrome c(Side 2) = quinone + 2 ferrocytochrome c(Side 2) + 2 H(+)(Side 2)

L-histidine-[translation elongation factor 2] + S-adenosyl-L-methionine = 2-((3S)-3-amino-3-carboxypropyl)-L-histidine-[translation elongation factor 2] + S-methyl-5'-thioadenosine

(8S)-3',8-cyclo-7,8-dihydroguanosine 5'-triphosphate = cyclic pyranopterin phosphate + diphosphate

ATP + D-ribulose 5-phosphate = ADP + D-ribulose 1,5-bisphosphate

D-glucose + O(2) = 2-dehydro-D-glucose + H(2)O(2)

Endonucleolytic cleavage to 5'-phosphomonoester.

Chloride + O(2) = chlorite

2-(N(omega)-L-arginino)succinate = fumarate + L-arginine

(S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate = fumarate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide

A pyrimidine 5'-nucleotide + H(2)O = D-ribose 5-phosphate + a pyrimidine base

(2E,6E)-farnesyl diphosphate + H(2)O = (2E,6E)-farnesol + diphosphate

(1a) 2 Fe(2+) + O(2) + 4 H(2)O = 2 (FeO(OH)) + 4 H(+) + H(2)O(2)

ATP + adenosine = ADP + AMP

(1a) L-cysteine + [enzyme]-cysteine = L-alanine + [enzyme]-S-sulfanylcysteine

Propane-1,2-diol = propanal + H(2)O

ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate

Trypanothione + NADP(+) = trypanothione disulfide + NADPH

CoA-(4'-phosphopantetheine) + apo-[acyl-carrier-protein] = adenosine 3',5'-bisphosphate + holo-[acyl-carrier-protein]

Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D-glucosyl)(n+1)

((1->4)-alpha-D-glucosyl)(n) + phosphate = ((1->4)-alpha-D-glucosyl)(n-1) + alpha-D-glucose 1-phosphate

ATP + UMP = ADP + UDP

ATP + 3-methylbut-3-en-1-yl phosphate = ADP + 3-methylbut-3-en-1-yl diphosphate

5,10-methylenetetrahydrofolate + dUMP + NADPH = dTMP + tetrahydrofolate + NADP(+)

(1a) S-adenosyl-L-methionine + L-histidine = S-adenosyl-L-homocysteine + N(alpha)-methyl-L-histidine

D-ribose 5-phosphate = D-ribulose 5-phosphate

L-aspartate + 2-oxoglutarate = oxaloacetate + L-glutamate

5-amino-6-(5-phospho-D-ribitylamino)uracil + NADP(+) = 5-amino-6-(5-phospho-D-ribosylamino)uracil + NADPH

ATP + 4-methyl-5-(2-hydroxyethyl)thiazole = ADP + 4-methyl-5-(2-phosphonooxyethyl)thiazole 

S-adenosyl-L-methionine + phospholipid olefinic fatty acid = S-adenosyl-L-homocysteine + phospholipid cyclopropane fatty acid

NH(3) + 2 H(2)O + 6 oxidized ferredoxin = nitrite + 6 reduced ferredoxin + 7 H(+)

ATP + (phosphate)(n) = ADP + (phosphate)(n+1)

ATP + AMP = 2 ADP

ATP + 6-hydroxymethyl-7,8-dihydropterin = AMP + 6-hydroxymethyl-7,8-dihydropterin diphosphate

ATP + (R)-5-diphosphomevalonate = ADP + phosphate + isopentenyl diphosphate + CO(2)

A (3R)-3-hydroxyacyl-[acyl-carrier-protein] + a UDP-3-O-((3R)-hydroxyacyl)-alpha-D-glucosamine = a UDP-2-N,3-O-bis((3R)-3-hydroxyacyl)-alpha-D-glucosamine + a holo-[acyl-carrier-protein]

(+)-trans-carveol + NAD(+) = (+)-(S)-carvone + NADH

GDP-beta-L-fucose + NADP(+) = GDP-4-dehydro-alpha-D-rhamnose + NADPH

An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate

2-phospho-D-glycerate = phosphoenolpyruvate + H(2)O

Protoheme + 3 [reduced NADPH--hemoprotein reductase] + 3 O(2) = biliverdin + Fe(2+) + CO + 3 [oxidized NADPH--hemoprotein reductase] + 3 H(2)O

(GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n)-diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = (GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala))(n+1)-diphosphoundecaprenol + undecaprenyl diphosphate

ATP + N-acetyl-L-glutamate = ADP + N-acetyl-L-glutamate 5-phosphate

UDP-alpha-D-glucose = UDP-alpha-D-galactose

Alpha-D-glucose = beta-D-glucose

D-ribulose 5-phosphate = D-xylulose 5-phosphate

(S)-4-hydroxy-2-oxohexanoate = propanal + pyruvate

Diphosphate + D-fructose 6-phosphate = phosphate + D-fructose 1,6-bisphosphate

ATP + kanamycin = ADP + kanamycin 3'-phosphate

L-phenylalanine + tetrahydrobiopterin + O(2) = L-tyrosine + 4a-hydroxytetrahydrobiopterin

sn-glycerol 3-phosphate + NAD(P)(+) = glycerone phosphate + NAD(P)H

ATP-dependent breakage, passage and rejoining of double-stranded DNA

Carbamoyl phosphate + N(2)-acetyl-L-ornithine = phosphate + N-acetyl-L-citrulline

Carbamoyl phosphate + L-aspartate = phosphate + N-carbamoyl-L-aspartate

L-asparagine + H(2)O = L-aspartate + NH(3)

A monocarboxylic acid amide + H(2)O = a monocarboxylate + NH(3)

(1a) S-adenosyl-L-methionine + [protein]-L-arginine = S-adenosyl-L-homocysteine + [protein]-N(omega)-methyl-L-arginine

Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-

A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H(2)O

ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole

A chalcone = a flavanone

(+)-muconolactone = cis,cis-muconate

2-carboxy-2,5-dihydro-5-oxofuran-2-acetate = cis,cis-butadiene-1,2,4-tricarboxylate

Geranyl diphosphate + isopentenyl diphosphate = diphosphate + (2E,6E)-farnesyl diphosphate

(1a) 2 cob(II)alamin + 2 [corrinoid adenosyltransferase] = 2 [corrinoid adenosyltransferase]-cob(II)alamin

6-hydroxymethyl-7,8-dihydropterin diphosphate + 4-aminobenzoate = diphosphate + dihydropteroate

The primary cleavage site is at 67-His-|-Lys-68 in human C5a with a minor secondary cleavage site at 58-Ala-|-Ser-59

Random hydrolysis of (1->4)-beta-D-mannosidic linkages in mannans, galactomannans and glucomannans

Random hydrolysis of (1->2)-glucosidic linkages in (1->2)-beta-D-glucans

ATP + thiamine phosphate = ADP + thiamine diphosphate

Acetyl-CoA + an alkane-alpha,omega-diamine = CoA + an N-acetyldiamine

(R)-3-hydroxybutanoate + NAD(+) = acetoacetate + NADH

(1a) acetyl-CoA + [acetyl-CoA C-acetyltransferase]-L-cysteine = [acetyl-CoA C-acetyltransferase]-S-acetyl-L-cysteine + CoA

L-ornithine = putrescine + CO(2)

S-adenosyl-L-methionine + 3-((1-carboxyvinyl)oxy)benzoate + H(2)O = 6-amino-6-deoxyfutalosine + L-methionine + HCO(3)(-)

(S)-malate + NADP(+) = pyruvate + CO(2) + NADPH

(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH

A primary alcohol + 2 cytochrome c(L) = an aldehyde + 2 reduced cytochrome c(L) + 2 H(+)

Reduced plastocyanin + oxidized ferredoxin + light = oxidized plastocyanin + reduced ferredoxin

Acetyl-CoA + H(2)O + oxaloacetate = citrate + CoA

A primary alcohol + 2 cytochrome c = an aldehyde + 2 reduced cytochrome c + 2 H(+)

ATP + protein L-histidine = ADP + protein N-phospho-L-histidine

4-amino-5-aminomethyl-2-methylpyrimidine + H(2)O = 4-amino-5-hydroxymethyl-2-methylpyrimidine + ammonia

ATP + glycerol = ADP + sn-glycerol 3-phosphate

UDP-alpha-D-glucose + D-glucose 6-phosphate = UDP + alpha,alpha-trehalose 6-phosphate

D-fructose 6-phosphate + phosphate = acetyl phosphate + D-erythrose 4-phosphate + H(2)O

A purine nucleoside + H(2)O = D-ribose + a purine base

L-ornithine = L-proline + NH(3)

(1a) D-serine = 2-aminoprop-2-enoate + H(2)O

Release of N-terminal amino acids, preferentially methionine, from peptides and arylamides.

IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate

dUTP + H(2)O = dUMP + diphosphate

7,8-dihydroneopterin 3'-triphosphate = 6-pyruvoyl-5,6,7,8-tetrahydropterin + triphosphate

(1a) [acetyl-CoA C-acyltransferase]-S-acyl-L-cyteine + acetyl-CoA = 3-oxoacyl-CoA + [acetyl-CoA C-acyltransferase]-L-cyteine

(9Z)-(13S)-12,13-epoxyoctadeca-9,11,15-trienoate = (15Z)-12-oxophyto-10,15-dienoate

Acetyl-CoA + enzyme N(6)-(dihydrolipoyl)lysine = CoA + enzyme N(6)-(S-acetyldihydrolipoyl)lysine

Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products

ATP + L-methionine + H(2)O = phosphate + diphosphate + S-adenosyl-L-methionine

4-amino-2-methyl-5-diphosphomethylpyrimidine + 2-((2R,5Z)-2-carboxy-4-methylthiazol-5(2H)-ylidene)ethyl phosphate = diphosphate + thiamine phosphate + CO(2)

Prenyl diphosphate + isopentenyl diphosphate = diphosphate + geranyl diphosphate

2,5-diamino-6-hydroxy-4-(5-phosphoribosylamino)pyrimidine + H(2)O = 5-amino-6-(5-phosphoribosylamino)uracil + NH(3)

3'-end directed exonucleolytic cleavage of viral RNA-DNA hybrid

ATP + D-fructose = ADP + D-fructose 6-phosphate

UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose

(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine

Hydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose and cellotetraose, releasing cellobiose from the non-reducing ends of the chains

5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO(2)

Vanillyl alcohol + O(2) = vanillin + H(2)O(2)

Hydrolysis of proteins and small molecule substrates at -Asn-|-Xaa- bonds.

Cellobiose = 4-O-beta-D-glucopyranosyl-D-mannose

L-leucine + 2-oxoglutarate = 4-methyl-2-oxopentanoate + L-glutamate

(2S)-2-hydroxy-2-methyl-3-oxobutanoate = (3R)-3-hydroxybutan-2-one + CO(2)

L-threonine + NAD(+) = L-2-amino-3-oxobutanoate + NADH

Isocitrate = succinate + glyoxylate

Endonucleolytic cleavage of DNA to give random double-stranded fragments with terminal 5'-phosphates, ATP is simultaneously hydrolyzed.

5,6,7,8-tetrahydrobiopterin + 2 NADP(+) = biopterin + 2 NADPH

3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholan-24-oylglycine + H(2)O = 3-alpha,7-alpha,12-alpha-trihydroxy-5-beta-cholanate + glycine

D-glucarate = 5-dehydro-4-deoxy-D-glucarate + H(2)O

2-dehydro-3-deoxy-6-phosphate-D-gluconate = pyruvate + D-glyceraldehyde 3-phosphate

UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose

L-fuculose 1-phosphate = glycerone phosphate + (S)-lactaldehyde

D-fructose 1,6-bisphosphate = glycerone phosphate + D-glyceraldehyde 3-phosphate

Maleate = fumarate

2 bilirubin + O(2) = 2 biliverdin + 2 H(2)O

(1a) 2 farnesyl diphosphate = diphosphate + presqualene diphosphate

D-glyceraldehyde 3-phosphate + phosphate + NAD(+) = 3-phospho-D-glyceroyl phosphate + NADH

Urea-1-carboxylate + H(2)O = 2 CO(2) + 2 NH(3)

(1a) 2 L-ascorbate + H(2)O(2) + 2 H(+) = 2 monodehydroascorbate + 2 H(2)O

Sedoheptulose 7-phosphate + D-glyceraldehyde 3-phosphate = D-ribose 5-phosphate + D-xylulose 5-phosphate

H(2) + 2 oxidized ferredoxin = 2 reduced ferredoxin + 2 H(+)

7-alpha,12-alpha-dihydroxy-3-oxochol-4-en-24-oyl-CoA = 12-alpha-hydroxy-3-oxochola-4,6-dien-24-oyl-CoA + H(2)O

Hydrolysis of dipeptides.

Adenosine + H(2)O = inosine + NH(3)

ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-methionyl-tRNA(Met)

NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]

ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-glutaminyl-tRNA(Gln)

NAD(+) + protein-L-arginine = nicotinamide + N(omega)-(ADP-D-ribosyl)-protein-L-arginine

Succinate + a quinone = fumarate + a quinol

ATP + L-lysine + tRNA(Lys) = AMP + diphosphate + L-lysyl-tRNA(Lys)

ATP + L-tryptophan + tRNA(Trp) = AMP + diphosphate + L-tryptophyl-tRNA(Trp)

ATP + acetyl-CoA + HCO(3)(-) = ADP + phosphate + malonyl-CoA

ATP + pyruvate + HCO(3)(-) = ADP + phosphate + oxaloacetate

Tetrahydropteroyl-(gamma-glutamyl)(n) + (n-1) H(2)O = 5,6,7,8-tetrahydrofolate + (n-1) L-glutamate

Catechol + O(2) = 2-hydroxymuconate-6-semialdehyde

(1a) (2R,3S)-3-isopropylmalate = 2-isopropylmaleate + H(2)O

Release of an N-terminal amino acid, Xaa-|-Yaa- from a peptide, amide or arylamide. Xaa is preferably Ala, but may be most amino acids including Pro (slow action). When a terminal hydrophobic residue is followed by a prolyl residue, the two may be released as an intact Xaa-Pro dipeptide.

Isochorismate + H(2)O = (2S,3S)-2,3-dihydroxy-2,3-dihydrobenzoate + pyruvate

Preferential release of a C-terminal lysine or arginine amino acid.

Myo-inositol hexakisphosphate + H(2)O = 1D-myo-inositol 1,2,4,5,6-pentakisphosphate + phosphate

L-lysine + NADPH + O(2) = N(6)-hydroxy-L-lysine + NADP(+) + H(2)O

An acylphosphate + H(2)O = a carboxylate + phosphate

A long-chain aldehyde + O(2) + 2 NADPH = an alkane + formate + H(2)O + 2 NADP(+)

L-cystathionine + H(2)O = L-homocysteine + NH(3) + pyruvate

Triacylglycerol + H(2)O = diacylglycerol + a carboxylate

A carboxylic ester + H(2)O = an alcohol + a carboxylate

(1a) L-cystathionine = L-cysteine + 2-aminobut-2-enoate

Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins

Thioredoxin + ROOH = thioredoxin disulfide + H(2)O + ROH

Hydrolysis of Xaa-|-Pro dipeptides. Also acts on aminoacyl-hydroxyproline analogs. No action on Pro-|-Pro.

(S)-dihydroorotate + fumarate = orotate + succinate

Sucrose = 6-O-alpha-D-glucopyranosyl-D-fructofuranose

N-isopropylammelide + H(2)O = cyanuric acid + isopropylamine

ATP = 3',5'-cyclic AMP + diphosphate

Cleaves -Ala-|-Ser- and -Ala-|-Ala- bonds in the scaffold protein.

Autocatalytic release of the core protein from the N-terminus of the togavirus structural polyprotein by hydrolysis of a -Trp-|-Ser- bond.

Autocatalytically cleaves itself from the polyprotein of the foot-and-mouth disease virus by hydrolysis of a Lys-|-Gly bond, but then cleaves host cell initiation factor eIF-4G at bonds -Gly-|-Arg- and -Lys-|-Arg-.

Hydrolysis of terminal (1->4)-linked alpha-D-glucose residues successively from non-reducing ends of the chains with release of beta-D-glucose

S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu)

(S)-malate = fumarate + H(2)O

1D-chiro-inositol + NAD(+) = 2D-2,3,5/4,6-pentahydroxycyclohexanone + NADH

(1a) L-glutamine + H(2)O = L-glutamate + NH(3)

Acetyl-CoA + kanamycin-B = CoA + N(6')-acetylkanamycin-B

Hydrolysis of terminal non-reducing alpha-L-arabinofuranoside residues in alpha-L-arabinosides

Exolytic cleavage of the (1->4)-beta-glycosidic linkage between N-acetylmuramic acid (MurNAc) and N-acetylglucosamine (GlcNAc) residues in peptidoglycan, from either the reducing or the non-reducing ends of the peptidoglycan chains, with concomitant formation of a 1,6-anhydrobond in the MurNAc residue.

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate

ATP + [biotin carboxyl-carrier protein]-biotin-N(6)-L-lysine + hydrogencarbonate- = ADP + phosphate + [biotin carboxyl-carrier protein]-carboxybiotin-N(6)-L-lysine

(S)-4-hydroxy-2-oxopentanoate = acetaldehyde + pyruvate

Malonyl-CoA + an [acyl-carrier-protein] = CoA + a malonyl-[acyl-carrier-protein]

(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O

CoB + CoM + methanophenazine = CoM-S-S-CoB + dihydromethanophenazine

Myo-inositol + NAD(+) = 2,4,6/3,5-pentahydroxycyclohexanone + NADH

ATP + H(2)O + K(+)(Side 1) = ADP + phosphate + K(+)(Side 2)

Glycerol + NAD(+) = glycerone + NADH

A primary alcohol + NAD(+) = an aldehyde + NADH

Hg + NADP(+) + H(+) = Hg(2+) + NADPH

Hydrolysis of terminal, non-reducing beta-D-mannose residues in beta-D-mannosides

ATP + L-histidine + tRNA(His) = AMP + diphosphate + L-histidyl-tRNA(His)

Hydrolysis of terminal non-reducing alpha-L-rhamnose residues in alpha-L-rhamnosides

4 ferrocytochrome c + O(2) + 4 H(+) = 4 ferricytochrome c + 2 H(2)O

NADPH + NAD(+) + H(+)(Side 1) = NADP(+) + H(+)(Side 2) + NADH