2xkk

X-ray diffraction
3.25Å resolution

CRYSTAL STRUCTURE OF MOXIFLOXACIN, DNA, and A. BAUMANNII TOPO IV (PARE-PARC FUSION TRUNCATE)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA topoisomerase 4 subunit A; DNA topoisomerase 4 subunit B Chains: A, C
Molecule details ›
Chains: A, C
Length: 767 amino acids
Theoretical weight: 86.17 KDa
Source organisms: Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: B0V9T6 (Residues: 370-627; Coverage: 41%)
  • Canonical: B0VP98 (Residues: 1-503; Coverage: 68%)
Gene names: ABAYE0126, ABSDF0217, parC, parE
Sequence domains:
Structure domains: Rossmann fold
DNA Chain: E
Molecule details ›
Chain: E
Length: 34 nucleotides
Theoretical weight: 10.49 KDa
DNA Chain: F
Molecule details ›
Chain: F
Length: 34 nucleotides
Theoretical weight: 10.42 KDa

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: C2
Unit cell:
a: 199.775Å b: 95.566Å c: 118.092Å
α: 90° β: 108.27° γ: 90°
R-values:
R R work R free
0.197 0.194 0.27
Expression system: Escherichia coli BL21(DE3)