2xjf

X-ray diffraction
2.1Å resolution

Crystal structure of the D52N variant of cytosolic 5'-nucleotidase II with a covalently modified Asn52

Released:

Function and Biology Details

Reaction catalysed:
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic purine 5'-nucleotidase Chain: A
Molecule details ›
Chain: A
Length: 555 amino acids
Theoretical weight: 64.13 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49902 (Residues: 1-536; Coverage: 96%)
Gene names: NT5B, NT5C2, NT5CP, PNT5
Sequence domains: 5' nucleotidase family

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: I222
Unit cell:
a: 92.095Å b: 128.222Å c: 130.439Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.181 0.178 0.225
Expression system: Escherichia coli BL21(DE3)