› 2xhz

X-ray diffraction
2.6Å resolution

Probing the active site of the sugar isomerase domain from E. coli arabinose-5-phosphate isomerase via X-ray crystallography

Released:
DOI: 10.2210/pdb2xhz/pdb
PDB entry 2xhz coloured by chain, front view.
PDB entry 2xhz coloured by chain, side view.
PDB entry 2xhz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Probing the active site of the sugar isomerase domain from E. coli arabinose-5-phosphate isomerase via X-ray crystallography.
Gourlay LJ, Sommaruga S, Nardini M, Sperandeo P, Dehò G, Polissi A, Bolognesi M
Protein Sci 19 2430-9 (2010)
PMID: 20954237

Function and Biology Details

Reaction catalysed: 
D-arabinose 5-phosphate = D-ribulose 5-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo tetramer
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-155278 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arabinose 5-phosphate isomerase KdsD Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 183 amino acids
Theoretical weight: 19.33 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P45395 (Residues: 1-183; Coverage: 56%)
Gene names: JW3164, b3197, kdsD, yrbH
Sequence domains: SIS domain
Structure domains: Glucose-6-phosphate isomerase like protein; domain 1

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: P21
Unit cell:
a: 55.86Å b: 67.43Å c: 82.17Å
α: 90° β: 106.94° γ: 90°
R-values:
R R work R free
0.263 0.261 0.305
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Conservation of the 2-keto-3-deoxymanno-octulosonic acid (Kdo) biosynthesis pathway between plants and bacteria.
Smyth et al. (2013)
12 other articles

2 mentions without citation

Probing the active site of the sugar isomerase domain from E. coli arabinose-5-phosphate isomerase via X-ray crystallography.
Gourlay et al. (2010)
1 more