2xgq

X-ray diffraction
2.7Å resolution

Structure of yeast DNA polymerase eta in complex with C8-N-acetyl-2- aminoanthracene containing DNA

Released:

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA polymerase eta Chains: A, B
Molecule details ›
Chains: A, B
Length: 536 amino acids
Theoretical weight: 60.71 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04049 (Residues: 1-513; Coverage: 81%)
Gene names: DBH1, RAD30, YDR419W
Sequence domains:
Structure domains:
5'-D(*GP*TP*GP*GP*AP*TP*GP*AP*G)-3' Chains: P, Q
Molecule details ›
Chains: P, Q
Length: 9 nucleotides
Theoretical weight: 2.84 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided
5'-D(*CP*8AG*CP*TP*CP*AP*TP*CP*CP*AP*C)-3' Chains: T, U
Molecule details ›
Chains: T, U
Length: 11 nucleotides
Theoretical weight: 3.49 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Not provided

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P41212
Unit cell:
a: 103.525Å b: 103.525Å c: 292.657Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.264
Expression systems:
  • Escherichia coli
  • Not provided