2xcx

X-ray diffraction
2.3Å resolution

Crystal structure of the apoform of the D52N variant of cytosolic 5'- nucleotidase II

Released:

Function and Biology Details

Reaction catalysed:
A 5'-ribonucleotide + H(2)O = a ribonucleoside + phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cytosolic purine 5'-nucleotidase Chain: A
Molecule details ›
Chain: A
Length: 554 amino acids
Theoretical weight: 63.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P49902 (Residues: 1-536; Coverage: 96%)
Gene names: NT5B, NT5C2, NT5CP, PNT5
Sequence domains: 5' nucleotidase family

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: I222
Unit cell:
a: 92.12Å b: 126.51Å c: 130.08Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.182 0.229
Expression system: Escherichia coli BL21(DE3)