2xcu

X-ray diffraction
2.42Å resolution

Membrane-embedded monofunctional glycosyltransferase WaaA of Aquifex aeolicus, complex with CMP

Released:

Function and Biology Details

Reaction catalysed:
A lipid IV(A) + CMP-beta-Kdo = an alpha-Kdo-(2->6)-lipid IV(A) + CMP
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
3-deoxy-D-manno-octulosonic acid transferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 374 amino acids
Theoretical weight: 43.28 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O66663 (Residues: 1-353; Coverage: 100%)
Gene names: aq_326, kdtA
Sequence domains: 3-Deoxy-D-manno-octulosonic-acid transferase (kdotransferase)
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-2
Spacegroup: P21
Unit cell:
a: 131.77Å b: 44.67Å c: 143.64Å
α: 90° β: 97.15° γ: 90°
R-values:
R R work R free
0.205 0.202 0.251
Expression system: Escherichia coli BL21