X-ray diffraction
2Å resolution

Membrane-embedded monofunctional glycosyltransferase WaaA of Aquifex aeolicus, substrate-free form


Function and Biology Details

Reaction catalysed:
A lipid IV(A) + CMP-beta-Kdo = an alpha-Kdo-(2->6)-lipid IV(A) + CMP
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
3-deoxy-D-manno-octulosonic acid transferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 374 amino acids
Theoretical weight: 43.28 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli BL21
  • Canonical: O66663 (Residues: 1-353; Coverage: 100%)
Gene names: aq_326, kdtA
Sequence domains: 3-Deoxy-D-manno-octulosonic-acid transferase (kdotransferase)
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I911-3
Spacegroup: P21
Unit cell:
a: 132.5Å b: 45.82Å c: 144.07Å
α: 90° β: 97.23° γ: 90°
R R work R free
0.21 0.207 0.254
Expression system: Escherichia coli BL21