2xa5

X-ray diffraction
1.09Å resolution

Structure of substrate binding protein SiaP (A11N) in complex with Neu5Ac

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sialic acid-binding periplasmic protein SiaP Chain: A
Molecule details ›
Chain: A
Length: 312 amino acids
Theoretical weight: 35.08 KDa
Source organism: Haemophilus influenzae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P44542 (Residues: 24-329; Coverage: 100%)
Gene names: HI_0146, siaP
Sequence domains: Bacterial extracellular solute-binding protein, family 7
Structure domains: Bacterial extracellular solute-binding protein, family 7

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P212121
Unit cell:
a: 47.818Å b: 74.7Å c: 87.254Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.119 0.118 0.144
Expression system: Escherichia coli BL21(DE3)