› 2x6w

X-ray diffraction
1.35Å resolution

Tailspike protein mutant E372Q of E.coli bacteriophage HK620 in complex with hexasaccharide

Released:
DOI: 10.2210/pdb2x6w/pdb
PDB entry 2x6w coloured by chain, front view.
PDB entry 2x6w coloured by chain, side view.
PDB entry 2x6w coloured by chain, top view.
Source organism: Enterobacteria phage HK620
Primary publication:
Single amino acid exchange in bacteriophage HK620 tailspike protein results in thousand-fold increase of its oligosaccharide affinity.
Broeker NK, Gohlke U, Müller JJ, Uetrecht C, Heinemann U, Seckler R, Barbirz S
Glycobiology 23 59-68 (2013)
PMID: 22923442

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189615 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Bacteriophage P22 tailspike N-terminal domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 600 amino acids
Theoretical weight: 64.7 KDa
Source organism: Enterobacteria phage HK620
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q9AYY6 (Residues: 111-710; Coverage: 85%)
Gene name: 9
Sequence domains: Phage spike trimer
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NDG, GLA, NAG, GLC, RAM
Ligand NDG 1 x NDG
Ligand GLA 1 x GLA
Ligand NAG 2 x NAG
Ligand GLC 2 x GLC
Ligand RAM 1 x RAM
2 bound ligands:
Ligand NA 3 x NA
Ligand TRS 1 x TRS
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P321
Unit cell:
a: 74.53Å b: 74.53Å c: 174.61Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.124 0.123 0.156
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Exploring the interactions between bacteriophage-encoded glycan binding proteins and carbohydrates.
Simpson et al. (2015)
8 other articles