› 2x67

X-ray diffraction
2.16Å resolution

The ternary complex of PrnB (the second enzyme in pyrrolnitrin biosynthesis pathway), tryptophan and cyanide

Released:
DOI: 10.2210/pdb2x67/pdb
PDB entry 2x67 coloured by chain, front view.
PDB entry 2x67 coloured by chain, side view.
PDB entry 2x67 coloured by chain, top view.
Source organism: Pseudomonas fluorescens
Primary publication:
The ternary complex of PrnB (the second enzyme in the pyrrolnitrin biosynthesis pathway), tryptophan, and cyanide yields new mechanistic insights into the indolamine dioxygenase superfamily.
Zhu X, van Pée KH, Naismith JH
J Biol Chem 285 21126-33 (2010)
PMID: 20421301

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161160 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Monodechloroaminopyrrolnitrin synthase PrnB Chain: A
Molecule details ›
Chain: A
Length: 361 amino acids
Theoretical weight: 39.92 KDa
Source organism: Pseudomonas fluorescens
Expression system: Pseudomonas fluorescens
UniProt:
  • Canonical: P95481 (Residues: 1-361; Coverage: 100%)
Gene name: prnB
Sequence domains: Monodechloroaminopyrrolnitrin synthase PrnB
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand HEM 1 x HEM
Ligand TRP 1 x TRP
Ligand SO4 2 x SO4
Ligand CYN 1 x CYN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: C2
Unit cell:
a: 68.392Å b: 79.441Å c: 92.058Å
α: 90° β: 103.17° γ: 90°
R-values:
R R work R free
0.168 0.166 0.211
Expression system: Pseudomonas fluorescens

Quick links

Citations

4 review citations

Applications of isothermal titration calorimetry in pure and applied research--survey of the literature from 2010.
Ghai et al. (2012)
3 more

2 mentions without citation

The ternary complex of PrnB (the second enzyme in the pyrrolnitrin biosynthesis pathway), tryptophan, and cyanide yields new mechanistic insights into the indolamine dioxygenase superfamily.
Zhu et al. (2010)
1 more