› 2x5n

X-ray diffraction
1.3Å resolution

Crystal Structure of the SpRpn10 VWA domain

Released:
DOI: 10.2210/pdb2x5n/pdb
PDB 2x5n coloured by chain and viewed from the front.
PDB 2x5n coloured by chain and viewed from the side.
PDB 2x5n coloured by chain and viewed from the top.
Source organism: Schizosaccharomyces pombe
Primary publication:
Structure of Rpn10 and its interactions with polyubiquitin chains and the proteasome subunit Rpn12.
Riedinger C, Boehringer J, Trempe JF, Lowe ED, Brown NR, Gehring K, Noble ME, Gordon C, Endicott JA
J. Biol. Chem. 285 33992-4003 (2010)
PMID: 20739285

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
26S proteasome regulatory subunit rpn10 Chain: A
Molecule details ›
Chain: A
Length: 192 amino acids
Theoretical weight: 21.36 KDa
Source organism: Schizosaccharomyces pombe
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O94444 (Residues: 2-193; Coverage: 79%)
Gene names: SPAC637.10c, pus1, rpn10
Sequence domains: von Willebrand factor type A domain
Structure domains: von Willebrand factor, type A domain

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2
Unit cell:
a: 79.99Å b: 35.23Å c: 60.14Å
α: 90° β: 111.2° γ: 90°
R-values:
R R work R free
0.125 0.123 0.165
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Mechanisms Regulating the UPS-ALS Crosstalk: The Role of Proteaphagy.
Quinet et al. (2020)
9 more

10 mentions without citation

Localization of the proteasomal ubiquitin receptors Rpn10 and Rpn13 by electron cryomicroscopy.
Sakata et al. (2012)
9 more