› 2x3j

X-ray diffraction
2Å resolution

CO-COMPLEX STRUCTURE OF ACHROMOBACTIN SYNTHETASE PROTEIN D (ACSD) WITH ATP AND N-CITRYL-ETHYLENEDIAMINE FROM PECTOBACTERIUM CHRYSANTHEMI

Released:
DOI: 10.2210/pdb2x3j/pdb
PDB entry 2x3j coloured by chain, front view.
PDB entry 2x3j coloured by chain, side view.
PDB entry 2x3j coloured by chain, top view.
Source organism: Dickeya chrysanthemi
Primary publication:
Structural basis for acyl acceptor specificity in the achromobactin biosynthetic enzyme AcsD.
Schmelz S, Botting CH, Song L, Kadi NF, Challis GL, Naismith JH
J Mol Biol 412 495-504 (2011)
PMID: 21835184

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-188037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
AcsD Chains: A, B
Molecule details ›
Chains: A, B
Length: 620 amino acids
Theoretical weight: 70.6 KDa
Source organism: Dickeya chrysanthemi
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93AT8 (Residues: 1-620; Coverage: 100%)
Gene name: acsD
Sequence domains:

Ligands and Environments

4 bound ligands:
Ligand ATP 2 x ATP
Ligand MG 2 x MG
Ligand GOL 5 x GOL
Ligand X3J 1 x X3J
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P1
Unit cell:
a: 57.704Å b: 71.521Å c: 95.595Å
α: 97.26° β: 101.97° γ: 91.01°
R-values:
R R work R free
0.192 0.19 0.231
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Ironing out siderophore biosynthesis: a review of non-ribosomal peptide synthetase (NRPS)-independent siderophore synthetases.
Carroll et al. (2018)
1 more

5 mentions without citation

Functional and structural analysis of the siderophore synthetase AsbB through reconstitution of the petrobactin biosynthetic pathway from Bacillus anthracis.
Nusca et al. (2012)
4 more