2x30

X-ray diffraction
1.95Å resolution

Function and Biology Details

Reactions catalysed:
N-(5-phospho-beta-D-ribosyl)anthranilate = 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose 5-phosphate
1-(5-phospho-beta-D-ribosyl)-5-((5-phospho-beta-D-ribosylamino)methylideneamino)imidazole-4-carboxamide = 5-((5-phospho-1-deoxy-D-ribulos-1-ylamino)methylideneamino)-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phosphoribosyl isomerase A Chain: A
Molecule details ›
Chain: A
Length: 240 amino acids
Theoretical weight: 25.06 KDa
Source organism: Streptomyces coelicolor
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P16250 (Residues: 1-240; Coverage: 100%)
Gene names: SC4G6.19c, SCO2050, hisA, priA
Structure domains: Aldolase class I

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P3121
Unit cell:
a: 63.6Å b: 63.6Å c: 102.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.216 0.273
Expression system: Escherichia coli BL21(DE3)