2x18

X-ray diffraction
1.46Å resolution

The crystal structure of the PH domain of human AKT3 protein kinase

Released:
Source organism: Homo sapiens
Entry authors: Vollmar M, Wang J, Zhang Y, Elkins JM, Burgess-Brown N, Chaikuad A, Pike ACW, von Delft F, Bountra C, Arrowsmith CH, Weigelt J, Edwards A, Knapp S

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RAC-gamma serine/threonine-protein kinase Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 119 amino acids
Theoretical weight: 14.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Y243 (Residues: 1-118; Coverage: 25%)
Gene names: AKT3, PKBG
Sequence domains: PH domain
Structure domains: Pleckstrin-homology domain (PH domain)/Phosphotyrosine-binding domain (PTB)

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P1
Unit cell:
a: 62.475Å b: 62.401Å c: 71.411Å
α: 111.47° β: 102.75° γ: 94.36°
R-values:
R R work R free
0.192 0.189 0.234
Expression system: Escherichia coli BL21(DE3)