› 2wzk

X-ray diffraction
2.05Å resolution

Structure of the Cul5 N-terminal domain at 2.05A resolution.

Released:
DOI: 10.2210/pdb2wzk/pdb
PDB entry 2wzk coloured by chain, front view.
PDB entry 2wzk coloured by chain, side view.
PDB entry 2wzk coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Molecular architecture of the ankyrin SOCS box family of Cul5-dependent E3 ubiquitin ligases.
Muniz JR, Guo K, Kershaw NJ, Ayinampudi V, von Delft F, Babon JJ, Bullock AN
J Mol Biol 425 3166-77 (2013)
PMID: 23806657

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-190056 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cullin-5 Chain: A
Molecule details ›
Chain: A
Length: 391 amino acids
Theoretical weight: 45.53 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9D5V5 (Residues: 1-386; Coverage: 50%)
Gene name: Cul5
Sequence domains: Cullin family
Structure domains: Cullin Repeats

Ligands and Environments

1 bound ligand:
Ligand EDO 5 x EDO
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I02
Spacegroup: P212121
Unit cell:
a: 30.18Å b: 64.5Å c: 197.54Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.19 0.188 0.224
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Targeting Cullin-RING E3 ubiquitin ligases for drug discovery: structure, assembly and small-molecule modulation.
Bulatov et al. (2015)
6 more

7 mentions without citation

Structural basis for Cul3 protein assembly with the BTB-Kelch family of E3 ubiquitin ligases.
Canning et al. (2013)
6 more