2wy2

Solution NMR

NMR structure of the IIAchitobiose-IIBchitobiose phosphoryl transition state complex of the N,N'-diacetylchitoboise brance of the E. coli phosphotransferase system.

Released:

Function and Biology Details

Reaction catalysed:
[Protein]-N(pi)-phospho-L-histidine + N,N'-diacetylchitobiose(Side 1) = [protein]-L-histidine + N,N'-diacetylchitobiose 6'-phosphate(Side 2)
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
PTS system N,N'-diacetylchitobiose-specific EIIA component Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 103 amino acids
Theoretical weight: 11.26 KDa
Source organism: Escherichia coli
Expression system: Milvago chimango chimango
UniProt:
  • Canonical: P69791 (Residues: 14-116; Coverage: 89%)
Gene names: JW1725, b1736, celC, chbA
Sequence domains: PTS system, Lactose/Cellobiose specific IIA subunit
Structure domains: Phosphotransferase system, lactose/cellobiose-type IIA subunit
PTS system N,N'-diacetylchitobiose-specific EIIB component Chain: D
Molecule details ›
Chain: D
Length: 103 amino acids
Theoretical weight: 11.07 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P69795 (Residues: 3-105; Coverage: 97%)
Gene names: JW1727, b1738, celA, chbB
Sequence domains: PTS system, Lactose/Cellobiose specific IIB subunit
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
Expression systems:
  • Milvago chimango chimango
  • Escherichia coli BL21(DE3)