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X-ray diffraction
1.8Å resolution

Clostridium perfringens alpha-toxin strain NCTC8237 mutant T74I

Released:

Function and Biology Details

Reaction catalysed:
A phosphatidylcholine + H(2)O = 1,2-diacyl-sn-glycerol + phosphocholine
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phospholipase C Chain: A
Molecule details ›
Chain: A
Length: 370 amino acids
Theoretical weight: 42.6 KDa
Source organism: Clostridium perfringens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0TV31 (Residues: 29-398; Coverage: 100%)
Gene names: CPF_0042, cpa, plc
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2
Spacegroup: C2221
Unit cell:
a: 59.861Å b: 173.643Å c: 78.564Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.177 0.175 0.216
Expression system: Escherichia coli