X-ray diffraction
1.5Å resolution

Crystal structure of the SidM/DrrA(GEF/GDF domain)-Rab1(GTPase domain) complex


Function and Biology Details

Reactions catalysed:
GTP + [protein]-L-tyrosine = diphosphate + [protein]-O(4)-(5'-guanylyl)-L-tyrosine
ATP + [protein]-L-threonine = diphosphate + [protein]-O(4)-(5'-adenylyl)-L-threonine
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ras-related protein Rab-1A Chain: A
Molecule details ›
Chain: A
Length: 175 amino acids
Theoretical weight: 19.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P62820 (Residues: 4-178; Coverage: 85%)
Gene names: RAB1, RAB1A
Sequence domains: Ras family
Structure domains: P-loop containing nucleotide triphosphate hydrolases
Multifunctional virulence effector protein DrrA Chain: B
Molecule details ›
Chain: B
Length: 217 amino acids
Theoretical weight: 24.29 KDa
Source organism: Legionella pneumophila
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q5ZSQ3 (Residues: 317-533; Coverage: 34%)
Gene names: drrA, lpg2464, sidM
Structure domains: Ferritin

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 68.88Å b: 73.803Å c: 73.665Å
α: 90° β: 90° γ: 90°
R R work R free
0.213 0.212 0.231
Expression system: Escherichia coli BL21(DE3)