2wwb

Electron Microscopy
6.48Å resolution

CRYO-EM STRUCTURE OF THE MAMMALIAN SEC61 COMPLEX BOUND TO THE ACTIVELY TRANSLATING WHEAT GERM 80S RIBOSOME

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentadecamer (preferred)
Entry contents:
11 distinct polypeptide molecules
4 distinct RNA molecules
Macromolecules (15 distinct):
Protein transport protein Sec61 subunit alpha isoform 1 Chain: A
Molecule details ›
Chain: A
Length: 476 amino acids
Theoretical weight: 52.28 KDa
Source organism: Canis lupus familiaris
UniProt:
  • Canonical: P38377 (Residues: 1-476; Coverage: 100%)
Gene name: SEC61A1
Sequence domains:
Protein transport protein Sec61 subunit gamma Chain: B
Molecule details ›
Chain: B
Length: 68 amino acids
Theoretical weight: 7.75 KDa
Source organism: Canis lupus familiaris
UniProt:
  • Canonical: P60058 (Residues: 1-68; Coverage: 100%)
Gene name: SEC61G
Sequence domains: SecE/Sec61-gamma subunits of protein translocation complex
Protein transport protein Sec61 subunit beta Chain: C
Molecule details ›
Chain: C
Length: 96 amino acids
Theoretical weight: 9.99 KDa
Source organism: Canis lupus familiaris
UniProt:
  • Canonical: P60467 (Residues: 1-96; Coverage: 100%)
Gene name: SEC61B
Sequence domains: Sec61beta family
60S ribosomal protein L4-B Chain: H
Molecule details ›
Chain: H
Length: 362 amino acids
Theoretical weight: 39.13 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P49626 (Residues: 1-362; Coverage: 100%)
Gene names: RPL2B, RPL4B, YD8119.17, YDR012W
Sequence domains:
60S ribosomal protein L17-A Chain: I
Molecule details ›
Chain: I
Length: 184 amino acids
Theoretical weight: 20.59 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P05740 (Residues: 1-184; Coverage: 100%)
Gene names: RPL17, RPL17A, RPL20A, YKL180W
Sequence domains: Ribosomal protein L22p/L17e
60S RIBOSOMAL PROTEIN L19 Chain: J
Molecule details ›
Chain: J
Length: 189 amino acids
Theoretical weight: 21.76 KDa
Source organism: Triticum aestivum
60S ribosomal protein L25 Chain: K
Molecule details ›
Chain: K
Length: 142 amino acids
Theoretical weight: 15.79 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P04456 (Residues: 1-142; Coverage: 100%)
Gene names: RPL25, YOL127W
Sequence domains:
60S ribosomal protein L26-A Chain: L
Molecule details ›
Chain: L
Length: 127 amino acids
Theoretical weight: 14.27 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P05743 (Residues: 1-127; Coverage: 100%)
Gene names: L8300.4, RPL26, RPL26A, RPL33A, YLR344W
Sequence domains:
60S ribosomal protein L31-A Chain: M
Molecule details ›
Chain: M
Length: 113 amino acids
Theoretical weight: 12.98 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P0C2H8 (Residues: 1-113; Coverage: 100%)
Gene names: D2478, RPL31A, RPL34, RPL34A, YDL075W
Sequence domains: Ribosomal protein L31e
60S ribosomal protein L35-A Chain: N
Molecule details ›
Chain: N
Length: 120 amino acids
Theoretical weight: 13.94 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P0CX84 (Residues: 1-120; Coverage: 100%)
Gene names: D1249, RPL35A, SOS1, YDL191W
Sequence domains: Ribosomal L29 protein
60S ribosomal protein L39 Chain: O
Molecule details ›
Chain: O
Length: 51 amino acids
Theoretical weight: 6.36 KDa
Source organism: Triticum aestivum
UniProt:
  • Canonical: P04650 (Residues: 1-51; Coverage: 100%)
Gene names: J0360, RPL39, RPL46, SPB2, YJL189W
Sequence domains: Ribosomal L39 protein
5.8S ribosomal RNA Chain: D
Molecule details ›
Chain: D
Length: 63 nucleotides
Theoretical weight: 20.3 KDa
Sequence domains: 5.8S ribosomal RNA
25S ribosomal RNA Chain: E
Molecule details ›
Chain: E
Length: 34 nucleotides
Theoretical weight: 11.09 KDa
25S ribosomal RNA Chain: F
Molecule details ›
Chain: F
Length: 25 nucleotides
Theoretical weight: 8.05 KDa
25S ribosomal RNA Chain: G
Molecule details ›
Chain: G
Length: 14 nucleotides
Theoretical weight: 4.48 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 6.48Å
Relevant EMDB volumes: EMD-1652