› 2wve

X-ray diffraction
2.3Å resolution

Structural and mechanistic insights into Helicobacter pylori NikR function

Released:
DOI: 10.2210/pdb2wve/pdb
PDB entry 2wve coloured by chain, front view.
PDB entry 2wve coloured by chain, side view.
PDB entry 2wve coloured by chain, top view.
Source organism: Helicobacter pylori 26695
Primary publication:
Structural and mechanistic insights into Helicobacter pylori NikR activation.
Bahlawane C, Dian C, Muller C, Round A, Fauquant C, Schauer K, de Reuse H, Terradot L, Michaud-Soret I
Nucleic Acids Res 38 3106-18 (2010)
PMID: 20089510

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Putative nickel-responsive regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 148 amino acids
Theoretical weight: 17.19 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O25896 (Residues: 1-148; Coverage: 100%)
Gene name: HP_1338
Sequence domains:
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand SO4 5 x SO4
Ligand GOL 3 x GOL
Ligand TRS 1 x TRS
Ligand CIT 1 x CIT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P43212
Unit cell:
a: 118.761Å b: 118.761Å c: 50.294Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.196 0.195 0.204
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Transcription factor-based biosensors enlightened by the analyte.
Fernandez-López et al. (2015)
8 more

2 mentions without citation

Structural and mechanistic insights into Helicobacter pylori NikR activation.
Bahlawane et al. (2010)
1 more