2wu8

X-ray diffraction
2.25Å resolution

Structural studies of phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv

Released:
Primary publication:
Structural studies of phosphoglucose isomerase from Mycobacterium tuberculosis H37Rv.
Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 66 490-7 (2010)
PMID: 20445242

Function and Biology Details

Reaction catalysed:
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chain: A
Molecule details ›
Chain: A
Length: 549 amino acids
Theoretical weight: 59.59 KDa
Source organism: Mycobacterium tuberculosis H37Rv
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P9WN69 (Residues: 7-553; Coverage: 99%)
Gene names: MTCY10D7.28, Rv0946c, pgi
Sequence domains: Phosphoglucose isomerase
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: I212121
Unit cell:
a: 109.7Å b: 118.5Å c: 137.6Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.183 0.182 0.223
Expression system: Escherichia coli BL21(DE3)