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Structure of Cerulean Fluorescent Protein at physiological pH

X-ray diffraction
1.15Å resolution
PDB entry 2wso coloured by chain, front view.
PDB entry 2wso coloured by chain, side view.
PDB entry 2wso coloured by chain, top view.
Source organism: Aequorea victoria
Assembly composition:
monomeric (preferred)
Entry contents: 1 distinct polypeptide molecule

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Assemblies

Assembly 1 (preferred)
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Assembly Name: Green fluorescent protein
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Multimeric state: monomeric
Accessible surface area: 9953.45 Å2
Buried surface area: 701.96 Å2
Dissociation area: 350.98 Å2
Dissociation energy (ΔGdiss): 0.8 kcal/mol
Dissociation entropy (TΔSdiss): 4.35 kcal/mol
Symmetry number: 1
PDBe Complex ID: PDB-CPX-154537

Macromolecules

Green fluorescent protein
Chain: A
Length: 237 amino acids
Theoretical weight: 26.79 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Pfam: Green fluorescent protein
InterPro:
CATH: Green fluorescent protein
Green fluorescent protein in PDB entry 2wso, assembly 1, front view.
Green fluorescent protein in PDB entry 2wso, assembly 1, side view.
Green fluorescent protein in PDB entry 2wso, assembly 1, top view.
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