› 2woj

X-ray diffraction
1.99Å resolution

ADP-AlF4 complex of S. cerevisiae GET3

Released:
DOI: 10.2210/pdb2woj/pdb
PDB entry 2woj coloured by chain, front view.
PDB entry 2woj coloured by chain, side view.
PDB entry 2woj coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The structural basis of tail-anchored membrane protein recognition by Get3.
Mateja A, Szlachcic A, Downing ME, Dobosz M, Mariappan M, Hegde RS, Keenan RJ
Nature 461 361-6 (2009)
PMID: 19675567

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171306 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ATPase GET3 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 354 amino acids
Theoretical weight: 39.39 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12154 (Residues: 1-354; Coverage: 100%)
Gene names: ARR4, D2371, GET3, YDL100C
Sequence domains: Anion-transporting ATPase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

4 bound ligands:
Ligand ADP 4 x ADP
Ligand ALF 4 x ALF
Ligand MG 4 x MG
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P21
Unit cell:
a: 53.612Å b: 82.628Å c: 167.282Å
α: 90° β: 98.28° γ: 90°
R-values:
R R work R free
0.178 0.176 0.213
Expression system: Escherichia coli

Quick links

Citations

29 review citations

Interactions between nuclei and the cytoskeleton are mediated by SUN-KASH nuclear-envelope bridges.
Starr et al. (2010)
28 more

28 mentions without citation

Tail-anchored membrane protein insertion into the endoplasmic reticulum.
Hegde et al. (2011)
27 more