› 2wkr

X-ray diffraction
2.2Å resolution

Structure of a photoactivatable Rac1 containing the Lov2 C450M Mutant

Released:
DOI: 10.2210/pdb2wkr/pdb
PDB entry 2wkr coloured by chain, front view.
PDB entry 2wkr coloured by chain, side view.
PDB entry 2wkr coloured by chain, top view.
Source organisms:
Primary publication:
A genetically encoded photoactivatable Rac controls the motility of living cells.
Wu YI, Frey D, Lungu OI, Jaehrig A, Schlichting I, Kuhlman B, Hahn KM
Nature 461 104-8 (2009)
PMID: 19693014

Function and Biology Details

Reactions catalysed: 
ATP + a protein = ADP + a phosphoprotein
GTP + H(2)O = GDP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129259 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ras-related C3 botulinum toxin substrate 1; non-specific serine/threonine protein kinase Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 37.63 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: O49003 (Residues: 404-546; Coverage: 16%)
  • Canonical: P63000 (Residues: 4-180; Coverage: 92%)
Gene names: MIG5, NPH1-1, RAC1, TC25
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand FMN 1 x FMN
3 bound ligands:
Ligand GTP 1 x GTP
Ligand MG 1 x MG
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P3221
Unit cell:
a: 112.39Å b: 112.39Å c: 69.2Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.188 0.186 0.226
Expression system: Escherichia coli

Quick links

Citations

222 review citations

Cell adhesion: integrating cytoskeletal dynamics and cellular tension.
Parsons et al. (2010)
221 more

1 mention without citation

A genetically encoded photoactivatable Rac controls the motility of living cells.
Wu et al. (2009)