2wk4

X-ray diffraction
2.98Å resolution

Dimeric structure of D347G D348G mutant of the sapporovirus RNA dependent RNA polymerase

Released:
Source organism: Sapporo virus
Entry authors: Fullerton SWB, Robel I, Schuldt L, Gebhardt J, Tucker PA, Rohayem J

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Endopeptidase with a preference for cleavage when the P1 position is occupied by Glu-|- and the P1' position is occupied by Gly-|-
NTP + H(2)O = NDP + phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Protease-polymerase p70 Chains: A, B
Molecule details ›
Chains: A, B
Length: 515 amino acids
Theoretical weight: 56.37 KDa
Source organism: Sapporo virus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q69014 (Residues: 1135-1649; Coverage: 23%)
Gene name: ORF1
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P41212
Unit cell:
a: 132.613Å b: 132.613Å c: 150.582Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.198 0.292
Expression system: Escherichia coli BL21