Structure analysis

Crystal structures of Holliday junction resolvases from Archaeoglobus fulgidus bound to DNA substrate

X-ray diffraction
1.8Å resolution
Assembly composition:
hetero tetramer (preferred)
Entry contents: 1 distinct polypeptide molecule
1 distinct DNA molecule

Assemblies

Assembly 1 (preferred)
Download    3D Visualisation
Multimeric state: hetero tetramer
Accessible surface area: 16000 Å2
Buried surface area: 5500 Å2
Dissociation area: 1,000 Å2
Dissociation energy (ΔGdiss): 4 kcal/mol
Dissociation entropy (TΔSdiss): 19 kcal/mol
Interface energy (ΔGint): -8 kcal/mol
Symmetry number: 1

Macromolecules

Chains: A, B
Length: 139 amino acids
Theoretical weight: 15.67 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28314 (Residues: 2-136; Coverage: 99%)
Gene names: AF_1965, hjc
Pfam: Archaeal holliday junction resolvase (hjc)
InterPro:
CATH: Trna Endonuclease; Chain: A, domain 1

Search similar proteins

Name: 5'-D(*DC*DG*DG*DA*DT*DA*DT*DC*DC*DGP)-3'
Representative chains: C, D
Source organism: synthetic construct [32630]
Expression system: Not provided
Length: 10 nucleotides
Theoretical weight: 3.05 KDa

Search similar DNA