PDBe 2wiw

X-ray diffraction
1.8Å resolution

Crystal structures of Holliday junction resolvases from Archaeoglobus fulgidus bound to DNA substrate

Released:
Entry authors: Carolis C, Koehler C, Sauter C, Basquin J, Suck D, Toeroe I

Function and Biology Details

Reaction catalysed:
Endonucleolytic cleavage at a junction such as a reciprocal single-stranded crossover between two homologous DNA duplexes (Holliday junction)
Biochemical function:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Holliday junction resolvase Hjc Chains: A, B
Molecule details ›
Chains: A, B
Length: 139 amino acids
Theoretical weight: 15.67 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O28314 (Residues: 2-136; Coverage: 99%)
Gene names: AF_1965, hjc
Sequence domains: Archaeal holliday junction resolvase (hjc)
Structure domains: Trna Endonuclease; Chain: A, domain 1
5'-D(*DC*DG*DG*DA*DT*DA*DT*DC*DC*DGP)-3' Chains: C, D
Molecule details ›
Chains: C, D
Length: 10 nucleotides
Theoretical weight: 3.05 KDa
Source organism: synthetic construct
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P212121
Unit cell:
a: 35.38Å b: 82.86Å c: 107.56Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.175 0.172 0.22
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided