Function and Biology Details
Reaction catalysed:
Cleavage of Arg-|-Ser bond in complement component C3 alpha-chain to yield C3a and C3b, and Arg-|- bond in complement component C5 alpha-chain to yield C5a and C5b.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
- Serine proteases, trypsin domain
- Peptidase S1A, chymotrypsin family
- von Willebrand factor, type A
- von Willebrand factor A-like domain superfamily
- Netrin module, non-TIMP type
- Tissue inhibitor of metalloproteinases-like, OB-fold
- Alpha-macroglobulin, receptor-binding
- Alpha-macroglobulin, receptor-binding domain superfamily
19 more domains
Structure analysis Details
Assembly composition:
hetero octamer (preferred)
PDBe Complex ID:
PDB-CPX-133310 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (12 distinct):
Complement C3 beta chain
Molecule details ›
Chains: A, C, E, G
Length: 645 amino acids
Theoretical weight: 71.39 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 645 amino acids
Theoretical weight: 71.39 KDa
Source organism: Homo sapiens
UniProt:
- Canonical:
P01024 (Residues: 23-667; Coverage: 39%)
Sequence domains:
- Macroglobulin domain MG1
- MG2 domain
- Macroglobulin domain MG3
- Macroglobulin domain MG4
- Alpha-2-macroglobulin bait region domain
Complement C3b alpha' chain
Molecule details ›
Chains: B, D, F, H
Length: 915 amino acids
Theoretical weight: 104.07 KDa
Source organism: Homo sapiens
UniProt:
Sequence domains:
Length: 915 amino acids
Theoretical weight: 104.07 KDa
Source organism: Homo sapiens
UniProt:
- Canonical: P01024 (Residues: 749-1663; Coverage: 56%)
Sequence domains:
- Alpha-2-macroglobulin family
- Complement component 3, CUB domain 1
- A-macroglobulin TED domain
- Complement component 3, CUB domain 2
- A-macroglobulin receptor binding domain
- UNC-6/NTR/C345C module
Complement factor B Bb fragment
Molecule details ›
Chains: I, J, K, L
Length: 507 amino acids
Theoretical weight: 57.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
Sequence domains:
Structure domains:
Length: 507 amino acids
Theoretical weight: 57.26 KDa
Source organism: Homo sapiens
Expression system: Homo sapiens
UniProt:
- Canonical: P00751 (Residues: 260-764; Coverage: 68%)
Sequence domains:
Structure domains:
Staphylococcal complement inhibitor
Molecule details ›
Chains: M, N, P, Q
Length: 92 amino acids
Theoretical weight: 10.78 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: Staphylococcal complement inhibitor SCIN
Structure domains: Substrate Binding Domain Of Dnak; Chain:A; Domain 2
Length: 92 amino acids
Theoretical weight: 10.78 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical: Q6GFB4 (Residues: 32-116; Coverage: 100%)
Sequence domains: Staphylococcal complement inhibitor SCIN
Structure domains: Substrate Binding Domain Of Dnak; Chain:A; Domain 2
Ligands and Environments
No modified residues
Experiments and Validation Details
X-ray source:
ESRF BEAMLINE ID14-4
Spacegroup:
P21
Expression systems:
- Homo sapiens
- Escherichia coli BL21(DE3)
