› 2wcs

X-ray diffraction
2.8Å resolution

Crystal Structure of Debranching enzyme from Nostoc punctiforme (NPDE)

Released:
DOI: 10.2210/pdb2wcs/pdb
PDB entry 2wcs coloured by chain, front view.
PDB entry 2wcs coloured by chain, side view.
PDB entry 2wcs coloured by chain, top view.
Source organism: Nostoc punctiforme
Primary publication:
Structural features of the Nostoc punctiforme debranching enzyme reveal the basis of its mechanism and substrate specificity.
Dumbrepatil AB, Choi JH, Park JT, Kim MJ, Kim TJ, Woo EJ, Park KH
Proteins 78 348-56 (2010)
PMID: 19768689

Function and Biology Details

Reaction catalysed: 
Cyclomaltodextrin + H(2)O = linear maltodextrin
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-109612 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glycosyl hydrolase family 13 catalytic domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 488 amino acids
Theoretical weight: 55.63 KDa
Source organism: Nostoc punctiforme
Expression system: Escherichia coli
UniProt:
  • Canonical: B2IUW9 (Residues: 1-488; Coverage: 100%)
Gene name: Npun_R6073
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P6122
Unit cell:
a: 93.731Å b: 93.731Å c: 257.042Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.224 0.224 0.284
Expression system: Escherichia coli

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Citations

1 review citation

Structure and function of α-glucan debranching enzymes.
Møller et al. (2016)
7 other articles