Electron Microscopy
9.4Å resolution

Kinesin-5-Tubulin Complex with AMPPNP

Primary publication:
9-Angström structure of a microtubule-bound mitotic motor.
J. Mol. Biol. 388 218-24 (2009)
PMID: 19285086
Related structures: EMD-1604

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Tubulin alpha-1D chain Chain: A
Molecule details ›
Chain: A
Length: 451 amino acids
Theoretical weight: 50.11 KDa
Source organism: Bos taurus
  • Canonical: Q2HJ86 (Residues: 1-452; Coverage: 100%)
Gene name: TUBA1D
Sequence domains:
Tubulin beta-2B chain Chain: B
Molecule details ›
Chain: B
Length: 445 amino acids
Theoretical weight: 49.91 KDa
Source organism: Bos taurus
  • Canonical: Q6B856 (Residues: 1-445; Coverage: 100%)
Gene name: TUBB2B
Sequence domains:
Kinesin-like protein Klp61F Chain: C
Molecule details ›
Chain: C
Length: 373 amino acids
Theoretical weight: 41.91 KDa
Source organism: Drosophila melanogaster
Expression system: Escherichia coli BL21(DE3)
  • Canonical: P46863 (Residues: 1-368; Coverage: 35%)
Gene names: CG9191, KLP2, Klp61F
Sequence domains: Kinesin motor domain

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 9.4Å
Relevant EMDB volumes: EMD-1604
Expression system: Escherichia coli BL21(DE3)