› 2w7n

X-ray diffraction
1.85Å resolution

Crystal Structure of KorA Bound to Operator DNA: Insight into Repressor Cooperation in RP4 Gene Regulation

Released:
DOI: 10.2210/pdb2w7n/pdb
PDB 2w7n coloured by chain and viewed from the front.
PDB 2w7n coloured by chain and viewed from the side.
PDB 2w7n coloured by chain and viewed from the top.
Source organism: Escherichia coli
Primary publication:
Crystal structure of KorA bound to operator DNA: insight into repressor cooperation in RP4 gene regulation.
König B, Müller JJ, Lanka E, Heinemann U
OpenAccess logo Nucleic Acids Res. 37 1915-24 (2009)
PMID: 19190096

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
TrfB transcriptional repressor protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 101 amino acids
Theoretical weight: 11.32 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P03052 (Residues: 1-101; Coverage: 100%)
Gene names: korA, trfB
Sequence domains: TrfB plasmid transcriptional repressor
Structure domains: Arc Repressor Mutant, subunit A
5'-D(*AP*AP*TP*GP*TP*TP*TP*AP*GP*CP *TP*AP*AP*AP*CP*AP*AP*G)-3' Chains: E, G
Molecule details ›
Chains: E, G
Length: 18 nucleotides
Theoretical weight: 5.55 KDa
Source organism: Escherichia coli
Expression system: Not provided
5'-D(*CP*BRUP*BRUP*GP*TP*TP*TP*AP*GP*CP*TP*AP *AP*AP*CP*AP*BRUP*T)-3' Chains: F, H
Molecule details ›
Chains: F, H
Length: 18 nucleotides
Theoretical weight: 5.68 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand BRU 6 x BRU

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P21
Unit cell:
a: 44.83Å b: 115.45Å c: 49.88Å
α: 90° β: 114° γ: 90°
R-values:
R R work R free
0.172 0.17 0.201
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

2 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
1 more

2 mentions without citation

Crystal structure of KorA bound to operator DNA: insight into repressor cooperation in RP4 gene regulation.
König et al. (2009)
1 more