› 2w4x

X-ray diffraction
2.42Å resolution

BtGH84 in complex with STZ

Released:
DOI: 10.2210/pdb2w4x/pdb
PDB entry 2w4x coloured by chain, front view.
PDB entry 2w4x coloured by chain, side view.
PDB entry 2w4x coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Primary publication:
Structural insight into the mechanism of streptozotocin inhibition of O-GlcNAcase.
He Y, Martinez-Fleites C, Bubb A, Gloster TM, Davies GJ
Carbohydr Res 344 627-31 (2009)
PMID: 19217614

Function and Biology Details

Reaction catalysed: 
[Protein]-3-O-(N-acetyl-beta-D-glucosaminyl)-L-serine + H(2)O = [protein]-L-serine + N-acetyl-D-glucosamine
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183580 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
O-GlcNAcase BT_4395 Chain: A
Molecule details ›
Chain: A
Length: 716 amino acids
Theoretical weight: 82.32 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q89ZI2 (Residues: 22-737; Coverage: 100%)
Gene name: BT_4395
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand GOL 1 x GOL
Ligand STZ 1 x STZ
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 186.99Å b: 52.344Å c: 84.267Å
α: 90° β: 99.3° γ: 90°
R-values:
R R work R free
0.185 0.182 0.235
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Challenges and issues with streptozotocin-induced diabetes - A clinically relevant animal model to understand the diabetes pathogenesis and evaluate therapeutics.
Goyal et al. (2016)
6 other articles

4 mentions without citation

The hexosamine signaling pathway: O-GlcNAc cycling in feast or famine.
Hanover et al. (2010)
3 more