› 2w4t

Electron Microscopy
35Å resolution

ISOMETRICALLY CONTRACTING INSECT ASYNCHRONOUS FLIGHT MUSCLE

Released:
DOI: 10.2210/pdb2w4t/pdb
PDB 2w4t coloured by chain and viewed from the front.
PDB 2w4t coloured by chain and viewed from the side.
PDB 2w4t coloured by chain and viewed from the top.
Source organism: Argopecten irradians
Primary publication:
Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions.
Wu S, Liu J, Reedy MC, Tregear RT, Winkler H, Franzini-Armstrong C, Sasaki H, Lucaveche C, Goldman YE, Reedy MK, Taylor KA
OpenAccess logo PLoS One 5 (2010)
PMID: 20844746
Related structures: EMD-1561

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Myosin heavy chain, striated muscle Chain: C
Molecule details ›
Chain: C
Length: 831 amino acids
Theoretical weight: 94.84 KDa
Source organism: Argopecten irradians
UniProt:
  • Canonical: P24733 (Residues: 5-835; Coverage: 43%)
Sequence domains: Myosin head (motor domain)
Myosin regulatory light chain, striated adductor muscle Chain: Y
Molecule details ›
Chain: Y
Length: 136 amino acids
Theoretical weight: 15.58 KDa
Source organism: Argopecten irradians
UniProt:
  • Canonical: P13543 (Residues: 16-151; Coverage: 87%)
Myosin essential light chain, striated adductor muscle Chain: Z
Molecule details ›
Chain: Z
Length: 151 amino acids
Theoretical weight: 17.05 KDa
Source organism: Argopecten irradians
UniProt:
  • Canonical: P07291 (Residues: 5-155; Coverage: 96%)
Sequence domains: EF-hand domain pair

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Resolution: 35Å
Relevant EMDB volumes: EMD-1561

Quick links

Citations

8 review citations

Insights into Actin-Myosin Interactions within Muscle from 3D Electron Microscopy.
Taylor et al. (2019)
7 more

2 mentions without citation

Electron tomography of cryofixed, isometrically contracting insect flight muscle reveals novel actin-myosin interactions.
Wu et al. (2010)
1 more