› 2w2f

X-ray diffraction
1.73Å resolution

CRYSTAL STRUCTURE OF SINGLE POINT MUTANT ARG48GLN OF P-COUMARIC ACID DECARBOXYLASE FROM LACTOBACILLUS PLANTARUM STRUCTURAL INSIGHTS INTO THE ACTIVE SITE AND DECARBOXYLATION CATALYTIC MECHANISM

Released:
DOI: 10.2210/pdb2w2f/pdb
PDB entry 2w2f coloured by chain, front view.
PDB entry 2w2f coloured by chain, side view.
PDB entry 2w2f coloured by chain, top view.
Source organism: Lactiplantibacillus plantarum
Primary publication:
p-Coumaric acid decarboxylase from Lactobacillus plantarum: structural insights into the active site and decarboxylation catalytic mechanism.
Rodríguez H, Angulo I, de Las Rivas B, Campillo N, Páez JA, Muñoz R, Mancheño JM
Proteins 78 1662-76 (2010)
PMID: 20112419

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123767 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenolic acid decarboxylase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 194 amino acids
Theoretical weight: 22.81 KDa
Source organism: Lactiplantibacillus plantarum
Expression system: Escherichia coli
UniProt:
  • Canonical: F9ULL2 (Residues: 1-178; Coverage: 100%)
Gene names: lp_3665, padA
Sequence domains: Phenolic acid decarboxylase (PAD)
Structure domains: Lipocalin

Ligands and Environments

1 bound ligand:
Ligand BA 2 x BA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM16
Spacegroup: P21
Unit cell:
a: 39.248Å b: 94.924Å c: 106.722Å
α: 90° β: 100.51° γ: 90°
R-values:
R R work R free
0.228 0.226 0.273
Expression system: Escherichia coli

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Citations

5 review citations

C-H Carboxylation of Aromatic Compounds through CO2 Fixation.
Luo et al. (2017)
4 more