2w27

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF THE BACILLUS SUBTILIS YKUI PROTEIN, WITH AN EAL DOMAIN, IN COMPLEX WITH SUBSTRATE C-DI-GMP AND CALCIUM

Released:

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Uncharacterized EAL-domain containing protein YkuI Chains: A, B
Molecule details ›
Chains: A, B
Length: 431 amino acids
Theoretical weight: 50.75 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O35014 (Residues: 1-407; Coverage: 100%)
Gene names: BSU14090, ykuI
Sequence domains: EAL-domain associated signalling protein domain
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 46.167Å b: 124.521Å c: 168.751Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.226 0.275
Expression system: Escherichia coli BL21(DE3)