2vwk

X-ray diffraction
2.6Å resolution

Uracil Recognition in Archaeal DNA Polymerases Captured by X-ray Crystallography. V93Q polymerase variant

Released:
Source organism: Thermococcus gorgonarius
Entry authors: Firbank SJ, Wardle J, Heslop P, Lewis RJ, Connolly BA

Function and Biology Details

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 773 amino acids
Theoretical weight: 89.97 KDa
Source organism: Thermococcus gorgonarius
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P56689 (Residues: 1-773; Coverage: 100%)
Gene names: pol, polA
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 58.432Å b: 104.747Å c: 154.408Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.217 0.275
Expression system: Escherichia coli BL21(DE3)